Click image to see more details
Product Info Summary
| SKU: | A00443-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Aim2 Antibody Picoband®
SKU/Catalog Number
A00443-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Aim2 Antibody Picoband® catalog # A00443-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Aim2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00443-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Aim2 recombinant protein (Position: Q136-E354).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00443-1 is reactive to Aim2 in Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
38.954kDa
Background of AIM2
Interferon-inducible protein AIM2,also known asabsent in melanoma 2or simplyAIM2,is aproteinthat in humans is encoded by theAIM2gene. It is mapped to 1q22. AIM2 is a member of theIfi202/IFI16family. It plays a putative role in tumorigenic reversion and may control cell proliferation.Interferon-gammainduces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNA(either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another proteinASC(or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains aCARD domain(caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation ofcaspase-1, an enzyme that processes proinflammatorycytokines(IL-1bandIL-18).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00443-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse RAW2647 whole cell, mouse NIH/3T3 whole cell
FCM: RAW2647 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Aim2 using anti-Aim2 antibody (A00443-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates,
Lane 2: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aim2 antigen affinity purified polyclonal antibody (Catalog # A00443-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aim2 at approximately 39 kDa. The expected band size for Aim2 is at 39 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of RAW264.7 cells using anti-Aim2 antibody (A00443-1).
Overlay histogram showing RAW264.7 cells stained with A00443-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aim2 Antibody (A00443-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Aim2 (Source: Uniprot.org, NCBI)
Gene Name
Aim2
Full Name
Interferon-inducible protein AIM2
Weight
38.954kDa
Superfamily
HIN-200 family
Alternative Names
Coagulation factor V; Activated protein C cofactor; Proaccelerin, labile factor; Coagulation factor V heavy chain; Coagulation factor V light chain; F5 AIM2 PYHIN4 absent in melanoma 2 interferon-inducible protein AIM2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Aim2, check out the Aim2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Aim2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aim2 Antibody Picoband® (A00443-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Aim2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Aim2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
