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Product Info Summary
| SKU: | A08482 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-AChR alpha10 CHRNA10 Antibody
View all Nicotinic Acetylcholine R alpha 10/CHRNA10 Antibodies
SKU/Catalog Number
A08482
Size
100μl
Form
Liquid
Description
Boster Bio Anti-AChR alpha10 CHRNA10 Antibody catalog # A08482. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AChR alpha10 CHRNA10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08482)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2503-2517 of Human PARC (C-terminus) coupled to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08482 is reactive to CHRNA10 in Human, Mouse, Rat
Applications
A08482 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
49705 MW
Background of Nicotinic Acetylcholine R alpha 10/CHRNA10
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. PARC is a cullin family member that functions as a cytoplasmic anchor protein in p53-associated protein complexes. PARC regulates the subcellular localization of p53 and subsequent function. PARC forms a complex with p53 in the cytoplasm of unstressed cells and interacts with UBCH7 and UBCH8. PARC shows a cytoplasmic localization and is ubiquitously expressed in all tissues with highest expression in testis brain and kidney.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using AChRalpha10 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using AChRalpha10 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For CHRNA10 (Source: Uniprot.org, NCBI)
Gene Name
CHRNA10
Full Name
Neuronal acetylcholine receptor subunit alpha-10
Weight
49705 MW
Superfamily
ligand-gated ion channel (TC 1.A.9) family
Alternative Names
cholinergic receptor, nicotinic, alpha 10; cholinergic receptor, nicotinic, alpha polypeptide 10; CHRNA10; NACHR alpha-10; NACHRA10; neuronal acetylcholine receptor subunit alpha-10; Nicotinic Acetylcholine R alpha 10; Nicotinic Acetylcholine Ra10; Nicotinic acetylcholine receptor subunit alpha-10 CHRNA10 cholinergic receptor nicotinic alpha 10 subunit neuronal acetylcholine receptor subunit alpha-10|NACHR alpha-10|acetylcholine receptor, nicotinic, alpha 10 (neuronal)|cholinergic receptor, nicotinic alpha 10|cholinergic receptor, nicotinic, alpha 10 (neuronal)|cholinergic receptor, nicotinic, alpha polypeptide 10|nicotinic acetylcholine receptor subunit alpha-10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHRNA10, check out the CHRNA10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHRNA10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AChR alpha10 CHRNA10 Antibody (A08482)
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No publications found for A08482
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-AChR alpha10 CHRNA10 Antibody
Question
I was wanting to use your anti-AChR alpha10 antibody for WB for human muscle of leg on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human muscle of leg identification?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
It shows on the product datasheet, A08482 anti-AChR alpha10 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human muscle of leg in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-24
Question
Is a blocking peptide available for product anti-AChR alpha10 antibody (A08482)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We do provide the blocking peptide for product anti-AChR alpha10 antibody (A08482). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-AChR alpha10 antibody A08482 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
S. Bhatt
Verified customer
Asked: 2019-03-13
Answer
The anti-AChR alpha10 antibody (A08482) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-13
Question
Will anti-AChR alpha10 antibody A08482 work on dog WB with muscle of leg?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
Our lab technicians have not validated anti-AChR alpha10 antibody A08482 on dog. You can run a BLAST between dog and the immunogen sequence of anti-AChR alpha10 antibody A08482 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog muscle of leg in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-18
Question
Is there a BSA free version of anti-AChR alpha10 antibody A08482 available?
Verified Customer
Verified customer
Asked: 2018-09-28
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-AChR alpha10 antibody A08482 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-28
Question
My question regarding product A08482, anti-AChR alpha10 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Zhang
Verified customer
Asked: 2015-03-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08482 anti-AChR alpha10 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-03-06
