Product Info Summary
SKU: | BA1045 |
---|---|
Size: | 10mg |
Host: | Rabbit |
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Product info
Product Name | AffiniPure Rabbit IgG (H+L) |
SKU/Catalog Number | BA1045 |
Pack Size | 10 mg |
Form | lyophilized |
Purity | The antibody was purified from antiserum by Protein A. |
Host | Rabbit |
Clone | Polyclonal |
Contents | 10 mg of affinity purified specific antibody (purification>99%). 0.01M PBS. |
Usage Method | Recommended Dilutions: Optimal working dilutions must be determined by end users. Working solution can not be preserved for long after being diluted with 0.01M PBS buffer solution (pH7.0-7.6) or 1% sodium acetate. After adding preservatives (such as 0.05% NaN3) and reagent grade BSA to 1%, the antibody can be preserved for a long time. When labeling antibodies, such as HRP, FITC, Biotin, etc., the reagents should not contain BSA and NaN3. |
Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. |
Applications | IHC, IF, ICC, WB, ELISA |
Cite This Product | Rabbit IgG (H+L) (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1045) |
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Validation Images & Assay Conditions
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Specific Publications For BA1045
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BA1045 has been cited in 42 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Andrographolide plays an important role in bleomycin-induced pulmonary fibrosis treatment
Exendin-4 protects retinal cells from early diabetes in Goto-Kakizaki rats by increasing the Bcl-2/Bax and Bcl-xL/Bax ratios and reducing reactive gliosis
Expression of toll-like receptor 4 in uvea-resident tissue macrophages during endotoxin-induced uveitis
Survivin is a prognostic indicator in glioblastoma and may be a target of microRNA‑218
c-Jun N-terminal kinase inhibitor favors transforming growth factor‑β to antagonize hepatitis B virus X protein‑induced cell growth promotion in hepatocellular carcinoma
Herpes Simplex Virus Type 2 Immediate Early Protein ICP27 Inhibits IFN-β Production in Mucosal Epithelial Cells by Antagonizing IRF3 Activation
Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4
Ets-2 Regulates Cell Apoptosis via the Akt Pathway, through the Regulation of Urothelial Cancer Associated 1, a Long Non-Coding RNA, in Bladder Cancer Cells
Exendin-4 Induces Bone Marrow Stromal Cells Migration Through Bone Marrow-Derived Macrophages Polarization via PKA-STAT3 Signaling Pathway
Protective Effect of an Antibody against Specific Extracellular Domain of TLR2 on Agonists-Driven Inflammatory and Allergic Response
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1 Customer Q&As for Rabbit IgG Isotype Control (H+L)
Question
In which range have you tested it? A customer is getting unspecified signals at a dilution of 1:700 (--> 1,4
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The suggested dilution ratio of BA1045 is 1:1000. The non-specific staining might be caused by the secondary antibody (2nd antibody: goat anti-rabbit IgG ) or the binding between Strep HRP and endogenous biotin in the sample. We suggest the customer run a negative control by using PBS to substitute the primary antibody to see if there is any non-specific staining.
Boster Scientific Support
Answered: 2019-11-08