RNA Purification Kit

SKU MB1006
Size 50 prep/250 prep
Reactivity Animal
Application RNA Purification, RNA Extraction

Description

Boster's RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from animal cells. The kit contains guanidine isothiocyanate and β‐mercaptoethanol and can inactivate the ribonucleases present in cell extracts. RNA in the sample is selectively absorbed onto the spin column and impurities are washed away. Total RNA is eluted from the column using RNase‐free water. The entire procedure takes approximately 15 minutes. 

Features of this kit include:  

• Fast. Using a rapid spin‐column format, the entire procedure takes approximately 15 minutes. 
• High Purity. OD260/OD280 ratio of purified RNA is generally > 1.9 
• Compatible with downstream applications such as Northern Blots, cDNA synthesis, RT‐PCR and qRT‐PCR. 
• Minimum RNA degradation. Guanidine isothiocyanate and β‐mercaptoethanol in the buffer maintains the integrity of the RNA 

Yield: 5‐15 μg total RNA can be purified from 25 mg animal ssue using this kit. Purified RNA is ready for most downstream applicaons such as RT‐PCR, Northern Blong, Poly (A) selecon and in vitro translaon.

Note before starting experiment

*Universal GT Solution and Universal NT Solution are supplied in a concentrated form. Before use, add 12ml or 60ml 96‐100% ethanol to 18 ml or 90ml concentrated universal GT solution and 24 ml 96‐100% ethanol to 6ml concentrated universal NT solution. 
NOTE: Care must be taken when working with RNA. It is important to maintain an RNAse‐free environment starting with RNA sample preparation and continue through purification and analysis. Use RNAse free tubes, tips, gels. Wear gloves at all times.  

Procedure

  • 1. Add 350 μl Buffer Rlysis‐AG into RNase‐Free 1.5 ml centrifuge tubes.
  • 2. Grind 25~50 mg animal tissue to fine powder in liquid nitrogen. Transfer the powder to the 1.5 ml RNase‐free centrifuge tube in step 1 and mix by inverting immediately.
  • 3. Incubate at room temperature for 5 minutes to make sure the cells are completely lysed.
  • 4. Add 1/2 volume of ethanol, mix by inverting the tube.
  • 5. Transfer the solution to the spin column. Centrifuge at 12,000 × g for 30 sec at room temperature, discard the flow‐through.
  • 6. Add 0.5 ml of Universal GT Solution to the column, centrifuge at 12,000 × g for 30 sec at room temperature, discard the flow‐through.
  • 7. Add 0.5 ml of Universal NT Solution to the column, centrifuge at 12,000 × g for 30 sec at room temperature, discard the flow‐through.
  • 8. Centrifuge the column at 12,000 × g for additional 30 sec at room temperature. Note: This step is very important to remove the residual ethanol thoroughly.
  • 9. Place the column in a new 1.5 ml RNase‐Free centrifuge tube. Add 50 μl RNase‐free Water. Keep at room temperature for 2 minutes. Centrifuge at 12,000 × g for 30 sec at room temperature, store RNA solution at ‐80°C.
  • Specifications

    SKU MB1006
    Size 50 prep/250 prep
    Reactivity Animal
    Application RNA Purification, RNA Extraction
    Storage & Handling 2 years at room temperature.
    Sample Type All animal tissues

    Kit Components

    Size 50 Preps 250 Preps
    Buffer Rlysis‐AG 25 ml 125 ml
    Universal GT Solution* 18 ml 90 ml
    Universal NT Solution* 6 ml 30 ml
    RNase‐free Water 5 ml 25 ml
    EZ‐10 Spin Column 50 250
    2 ml Collection Tube 50 250
    Protocol 1 1

    Materials Needed But Not Provided

    • Microcentrifuge capable of at least 12,000 × g
    • RNase‐Free pipets and pipet ps
    • Vortexer
    • RNase‐Free Ethanol (96‐100%)
    • RNase‐Free Microcentrifuge tubes (1.5ml or 2ml)
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    MB1006
    $100.00

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