Electrophoresis/Gel Staining

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  1. Ponceau S solution AR0142
    $10.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining
    • 12ml
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    Ponceau S solution can be used for rapid staining of protein bands on PVDF and nitrocellulose membrane. Since the dye of this reagent is with negative charge, it can combine with amino-acid residue with positive charge. Meanwhile, it also can combine with non-polar area of protein to form red bands thereby. For the reversible protein staining, protein can be cleaned again by washing with distilled water, PBS buffer or other appropriate solution. With advantages of convenient use, low background and high sensitivity, this product can detect protein at minimum 250ng, but it is not suitable for protein detection on nylon membrane. Learn More
  2. Silver Stain kit AR0171
    $100.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining, Total Protein Analysis
    • 1kit
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    Silver Staining Kit uses ammoniacal silver chemistry and glutaraldehyde sensitization to produce a highly sensitive silver stain, capable of detecting much lower levels of protein than standard Coomassie or Colloidal Blue techniques. Clear background makes sample identification unambiguous and provides publication-quality gels. With the advantages of easy and effective operation, Boster's Silver Stain kit is suitable for the SDS-PAGE or non-denaturing PAGE. It is suitable for staining both single dimension SDS-PAGE gels and two-dimensional (2D) gels of complex protein solutions, and the staining gel will be compatible with subsequent mass spectrometry detection. The sensitivity of silver staining is 100 times as high as that of CBB (Coomassie brilliant blue) staining. The kit can detect 0.25 ng BSA. It is unnecessary to use the toxic methanol. Learn More
  3. Coomassie Blue Fast Staining Solution AR0170
    $30.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining, Total Protein Analysis
    • 1kit
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    Boster’s Commassie Blue Fast Staining Solution is used for rapid staining of protein on polyacrylamide gels. Learn More
  4. Coomassie Blue Staining Destaining Solution AR0140
    $20.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining, Total Protein Analysis
    • 1kit
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    Boster’s Commassie Blue Staining Destaining Solution is used for staining of protein on polyacrylamide gels. Learn More
  5. SDS-PAGE Gel Preparation Kit AR0138
    $45.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining
    • 1kit (for 30-50 gels)
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    Boster’s SDS-PAGE Gel Preparation Kit, Western Blotting Related Reagent. Learn More
  6. Tris-Glycine SDS Running Buffer Pack AR0139
    $5.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining
    • 1L (powder)
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    Boster’s Tris-Glycine SDS Running Buffer Pack is a pouch of dry-blend powder that is sufficient to make 1L of Tris-Glycine SDS Running Buffer for SDS-PAGE (protein polyacrylamide gel electrophoresis). Dry-Blend Packs of Tris-glycine-SDS buffer are easy to use. Simply empty contents of one envelope pack into a beaker, add distilled water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 1 L of water, each pack makes 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3. Learn More
  7. Dual Color protein Loading Buffer 4X AR1142
    $15.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining
    • 1mL X 6 (sufficient to make 24mL of protein solution)
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    Boster’s Dual Color Protein Loading Buffer 4X is a complete solution for the preparation of protein samples prior to SDS-PAGE and monitoring protein transfer during Western blotting. Learn More
  8. SDS-PAGE Protein Loading Buffer 5X (Reducing) AR1112
    $10.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining
    • 3mL
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    SDS-PAGE Protein Loading Buffer 5X (Reducing) Learn More
  9. SDS-PAGE Protein Loading Buffer 2X (Reducing) AR0131
    $10.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining
    • 6ml
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    SDS PAGE Sample Buffer 2X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system. Learn More
  10. Tricine SDS Sample Buffer 2X AR1143
    $15.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining
    • 6ml
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    Tricine SDS Sample Buffer 2X is used for loading low molecular weight proteins and peptides for SDS-PAGE analysis on polyacrylamide gels. Learn More

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SDS-Polyacrylamide Gel Electrophoresis Kits and Reagents

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Introduction

Once your protein sample has been extracted, prepared, and quantified, it is time to perform gel electrophoresis. With proper preparation and execution, gel electrophoresis will cleanly separate the proteins by size, allowing you to measure not only your protein’s presence, but it’s relative size as well. Boster provides SDS-PAGE Gel Preparation kits and other high-quality reagents so you can get the most out of your samples.

Procedure

To prepare your sample for electrophoresis, mix the sample with Boster dual color protein loading buffer and denature the resulting mixture in a 100°C water bath. Once your sample is ready, fill your electrophoresis chamber with enough Boster electrophoresis buffer to cover the gel. Carefully pipette your sample into the wells, using enough volume to ensure consistent amounts of protein, and making sure not to poke the pipette tip into the gel. Connect your electrophoresis chamber to the power supply of your choice, and run the gel for enough time to adequately separate your protein sample.

After your gel has run for an appropriate amount of time, us one of Boster’s protein visualization reagents to verify the success and quality of your electrophoresis experiment.

For a detailed protocol for gel electrophoresis, see our Western blot handbook.

Tips

  1. For better results, optimize the gel concentration, SDS concentration, and run time and temperature prior to running the gel
  2. Ensure that each well has the same amount of protein before running the gel
  3. If you encounter streaked, distorted, or smile bands, check your electrophoresis parameters. See our troubleshooting guides for more information
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