Chromogenic Development



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  1. WB Developing Fixing Kit AR0132
    $25.00
    • Western Blotting Reagents, Chromogenic Development
    • 1kit
    • boster_tick.pngShips next business day
    Boster’s WB Developing Fixing Kit is a processing kit, which can be used in Western, Northern and Southern Blotting after squashed. This kit is especially suitable for X-ray developing and fixing, as well as normal black & white negative film or photograph. Learn More
  2. BCIP/ NBT Chromogenic Substrate Kit AR1023
    $52.00
    • Western Blotting Reagents, Chromogenic Development
    • 1kit
    • boster_tick.pngShips next business day
    Boster’s BCIP/ NBT Chromogenic Substrate Kit is ready-made solutions for chromogenic blot and immunohistochemical staining applications. Learn More

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Western Blotting Chromogenic/Chemiluminescent Development Products

antibody pairing

Introduction

The final step of Western blotting is detection; after the enzyme-linked secondary antibody has bound, adding the corresponding substrate causes a change in color or fluorescence that signals the presence of the target protein.

Procedure

After preparing the chromogenic or chemiluminescent substrate according to the instructions on the datasheet, cover the membrane with the substrate and incubate on a shaker until bands appear. Rinse the membrane and cover with film or a glass cover. Photograph the gel or expose the film and enjoy the excellent results of using Boster products.

For a detailed protocol for signal development, see our Western blot handbook.

Tips

Chemiluminescent development tends to be more sensitive, and more expensive than chromogenic development. Be sure to pick the right method for your needs.

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