Chemiluminescent Development



Page 1 of 1

per page
  1. ECL Plus Western Blotting Substrate AR1196
    $50.00
    • Western Blotting Reagents, Chemiluminescent Development
    • 50mL
    • boster_tick.pngShips next business day
    Boster's ECL Plus Western Blotting Substrate is an ultra-sensitive, luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) at high sensitivity levels (low picogram to mid-femtogram). Boster Western Blotting Substrate may be used for immunoblots, western blots, dot blots and any blotting application utilizing horseradish peroxidase (HRP)-conjugates. The substrate can be used with various blocking buffers and on nitrocellulose or PVDF membranes. Such blots will exhibit low backgrounds. Produced chemiluminescence can be visualized on CCD imaging systems or x-ray film. Learn More
  2. Hypersensitive WB Chemiluminescent Substrate CR0001-25
    $260.00
    • Western Blotting Reagents, Chemiluminescent Development
    • 50mL (sufficient reagents for 10000 cm2 of membrane)
    • boster_tick.pngShips next business day
    Boster’s Hypersensitive Western Blot Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram). Learn More
  3. Hypersensitive WB Chemiluminescent Substrate CR0001-10
    $115.00
    • Western Blotting Reagents, Chemiluminescent Development
    • 20mL (sufficient reagents for 4000 cm2 of membrane)
    • boster_tick.pngShips next business day
    Boster’s Hypersensitive Western Blot Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram). Learn More
  4. Hypersensitive WB Chemiluminescent Substrate CR0001-5
    $65.00
    • Western Blotting Reagents, Chemiluminescent Development
    • 10mL (sufficient reagents for 2000 cm2 of membrane)
    • boster_tick.pngShips next business day
    Boster’s Hypersensitive Western Blot Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram). Learn More
  5. ECL Western Blotting Substrate AR1170
    $98.00
    • Western Blotting Reagents, Chemiluminescent Development
    • 200mL
    • boster_tick.pngShips next business day
    Hypersensitive ECL chemiluminiscence substrate, Western Blotting Related Reagent Learn More

Page 1 of 1

per page

Western Blotting Chromogenic/Chemiluminescent Development Products

antibody pairing

Introduction

The final step of Western blotting is detection; after the enzyme-linked secondary antibody has bound, adding the corresponding substrate causes a change in color or fluorescence that signals the presence of the target protein.

Procedure

After preparing the chromogenic or chemiluminescent substrate according to the instructions on the datasheet, cover the membrane with the substrate and incubate on a shaker until bands appear. Rinse the membrane and cover with film or a glass cover. Photograph the gel or expose the film and enjoy the excellent results of using Boster products.

For a detailed protocol for signal development, see our Western blot handbook.

Tips

Chemiluminescent development tends to be more sensitive, and more expensive than chromogenic development. Be sure to pick the right method for your needs.

Copyright © 2019 Boster Biological Technology Inc. All rights reserved.