Mouse recombinant IFN alpha 1a (Interferon alpha 1a) protein, AF

IFN-alpha 1 protein, Mouse

Interferon Alpha 1a (IFN alpha 1a) is a leukocyte interferon, which is a variant of Interferon-alpha. IFN-alpha 1a is a 18.4 kDa protein containing 166 amino acid residues. It could bind the interferon receptors that activates the signal transduction of immune responses through the Jak/STAT pathway in leukocytes and lymphoblastoid cells.

Product Info Summary

SKU: PROTP01572
Size: 5ug,20ug,100ug
Origin Species: Mouse
Source: Escherichia coli
Application: Cell Culture

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Product Name

Mouse recombinant IFN alpha 1a (Interferon alpha 1a) protein, AF

View all IFN-alpha 1 recombinant proteins

SKU/Catalog Number

PROTP01572

Size

5ug,20ug,100ug

Tag

His Tag (N-term)

Description

Interferon Alpha 1a (IFN alpha 1a) is a leukocyte interferon, which is a variant of Interferon-alpha. IFN-alpha 1a is a 18.4 kDa protein containing 166 amino acid residues. It could bind the interferon receptors that activates the signal transduction of immune responses through the Jak/STAT pathway in leukocytes and lymphoblastoid cells.

Storage & Handling

Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.

Cite This Product

Mouse recombinant IFN alpha 1a (Interferon alpha 1a) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP01572)

Form

Lyophilized

Formulation

The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.

Purity

>98% as determined by SDS-PAGE.

Predicted MW

21.725kDa

Molecular weight

The protein has a calculated MW of 19.93 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).

Activity

Measure by its ability to protect L929 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <10 pg/mL. The specific activity of recombinant mouse IFN alpha 1a is > 4 x 10⁷ IU/mg.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Amino Acid Sequence

CDLPQTHNLRNKRALTLLVQMRRLSPLSCLKDRKDFGFPQEKVDAQQIKKAQAIPVLSELTQQILNIFTSKDSSAAWNTTLLDSFCNDLHQQLNDLQGCLMQQVGVQEFPLTQEDALLAVRKYFHRITVYLREKKHSPCAWEVVRAEVWRALSSSANVLGRLREEK with polyhistidine tag at the N-terminus.

Reconstitution

Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

Validation Images & Assay Conditions

Gene/Protein Information For Ifna1 (Source: Uniprot.org, NCBI)

Gene Name

Ifna1

Full Name

Interferon alpha-1/13

Weight

21.725kDa

Superfamily

alpha/beta interferon family

Alternative Names

Ifa, Ifa8 IFNA1 IFL, IFN, IFN-ALPHA, IFN-alphaD3, IFNA@, leIF D, IFNA1 interferon alpha 1 interferon alpha-1/13|IFN-alpha-1/13|interferon alpha 1b|interferon alpha-D|interferon-alpha1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on Ifna1, check out the Ifna1 Infographic

Ifna1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ifna1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $77

SKU:PROTP01572

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