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Product Info Summary
| SKU: | EK1978 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse FCRL5 ELISA Kit PicoKine®
View all FCRL5/FcRH5/IRTA2 ELISA kits
SKU/Catalog Number
EK1978
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse FCRL5 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse FCRL5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse FCRL5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1978)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0, Immunogen sequence: Q27-A496
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1978 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1978 is reactive to FCRL5 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1978 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCRL5/FcRH5/IRTA2
Fc receptor-like protein 5 is a protein that in humans is encoded by the FCRL5 gene. It is mapped to 3; 3 F1. FCRL5 has also been designated as CD307 (cluster of differentiation 307).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1978-CAP | Anti-Mouse FCRL5 Pre-coated 96-well strip microplate | 1 |
| EK1978-ST | Mouse FCRL5 Standard | 2 vials, 10 ng/tube |
| EK1978-DA | Mouse FCRL5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.121 | 0.544 | 0.689 | 0.950 | 1.381 | 1.720 | 2.028 | 2.179 |
Data Example Images
Click image to see more details
Mouse FCRL5 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FCRL5/FcRH5/IRTA2, Dilution ratio of 1:1, concentration in serum and plasma is 100-200 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse FCRL5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 18 | 72 | 133 | 22 | 85 | 154 |
| Standard deviation | 1.06 | 3.17 | 8.38 | 1.01 | 4.93 | 11.55 |
| CV (%) | 5.9% | 4.4% | 6.3% | 4.6% | 5.8% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FCRL5/FcRH5/IRTA2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 18 | 22 | 28 | 29 | 24 | 1.62 | 6.7% |
| Sample 2 | 72 | 78 | 85 | 92 | 82 | 4.33 | 5.3% |
| Sample 3 | 133 | 145 | 1.57 | 144 | 145 | 7.09 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For FCRL5 (Source: Uniprot.Org, NCBI)
Gene Name
FCRL5
Full Name
Fc receptor-like protein 5
Weight
106.437kDa
Alternative Names
Fc receptor-like protein 5; FcR-like protein 5; FcRL5; BXMAS1-like protein 2; Mbxmh2; Fc receptor homolog 3; FcRH3; moFcRH3; CD307e; CD307e; Fcrl5; Fcrh3 FCRL5 BXMAS1, CD307, CD307e, FCRH5, IRTA2, PRO820 Fc receptor like 5 Fc receptor-like protein 5|fc receptor homolog 5|fcR-like protein 5|immune receptor translocation-associated protein 2|immunoglobulin superfamily receptor translocation associated 2 (IRTA2)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FCRL5, check out the FCRL5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCRL5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse FCRL5 ELISA Kit PicoKine® (EK1978)
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Customer Q&As
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5 Customer Q&As for Mouse FCRL5 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for FCRL5 ELISA kit? I used your FCRL5 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain FCRL5 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-07-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FCRL5 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of FCRL5 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-07-05
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-04-17
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Clark
Verified customer
Asked: 2019-12-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-12-05
Question
Q: how to thaw whole blood sample for FCRL5 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-09-14
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FCRL5 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-09-14
Question
Q: can I use citrate plasma as samples in Mouse FCRL5 Picokine® ELISA Kit (Catalog # EK1978)?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of FCRL5 causing degradation of its protein structure. FCRL5 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the FCRL5 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-04-11
