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Product Info Summary
| SKU: | PROTP50591-6 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant TRAIL (TNF-related apoptosis-inducing ligand) protein, AF
View all TRAIL/TNFSF10 recombinant proteins
SKU/Catalog Number
PROTP50591-6
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Tumor necrosis factor?associated apoptosis?inducing ligand (TRAIL) is a type II membrane protein of the tumor necrosis factor (TNF) family members, moreover, expressed in many adult tissues including the thymus, prostate, colon, ovary and lung. TRAIL is a 19kDa protein containing 281 residues. TRAIL to induce apoptosis in human breast carcinoma cells (MCF7) and human epitheloid carcinoma (HeLa) cell lines, by activate two death receptors of DR4 and DR5 or two decoy receptors DcR1 and DcR2.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant TRAIL (TNF-related apoptosis-inducing ligand) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP50591-6)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Predicted MW
32.509kDa
Molecular weight
The protein has a calculated MW of 20.33 kDa. The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is 10.4-15.4 ng/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human TRAIL
Protein Target Info & Infographic
Gene/Protein Information For TNFSF10 (Source: Uniprot.org, NCBI)
Gene Name
TNFSF10
Full Name
Tumor necrosis factor ligand superfamily member 10
Weight
32.509kDa
Superfamily
tumor necrosis factor family
Alternative Names
TNFSF10, Apo2 Ligand, TL2, Apo2L, CD253 TNFSF10 APO2L, Apo-2L, CD253, TL2, TNLG6A, TRAIL TNF superfamily member 10 tumor necrosis factor ligand superfamily member 10|Apo-2 ligand|TNF-related apoptosis inducing ligand TRAIL|chemokine tumor necrosis factor ligand superfamily member 10|tumor necrosis factor (ligand) family, member 10|tumor necrosis factor (ligand) superfamily, member 10|tumor necrosis factor apoptosis-inducing ligand splice variant delta|tumor necrosis factor ligand 6A|tumor necrosis factor superfamily member 10
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TNFSF10, check out the TNFSF10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFSF10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant TRAIL (TNF-related apoptosis-inducing ligand) protein, AF (PROTP50591-6)
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