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Product Info Summary
| SKU: | PROTQ02297-3 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human |
| Source: | Escherichia coli |
| Application: | Cell Culture |
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Product info
Product Name
Human recombinant Heregulin Beta 1 protein, AF
View all NRG1 recombinant proteins
SKU/Catalog Number
PROTQ02297-3
Size
5ug,20ug,100ug
Tag
His Tag (C-term)
Description
Neuregulin-1 (NRG-1, also called heuregulin1 or neu differentiation factor) is a glycoprotein that belongs to the neuregulins family. Structurally, Neuregulin-1 harbors tissue-speci?c N terminal sequence, followed by immunoglobulin-like (Ig-like) domains, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. NRG1 has multiple isoforms produced by alternative splicing. Heregulin- β1 (HRG- β1) is one of the isoforms, has been reported to engage the development and survival of cardiomyocytes derived from embryonic stem (ES) cells via activating MAPK-ERK and PI3K-AKT pathways. Moreover, HRG- β1 plays a central role in promoting the proliferation of neuronal progenitors from embryonic neural stem cells.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human recombinant Heregulin Beta 1 protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ02297-3)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Predicted MW
70.392kDa
Molecular weight
The protein has a calculated MW of 8.42 kDa. The protein migrates as 8 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce MCF-7 cells proliferation. The ED₅₀ for this effect is < 10 ng/mL.
Endotoxin
<1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
A DNA sequence encoding Human Heregulin Beta 1 Protein (#Q02297-6)(Ser177-Glu241) was expressed with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human Heregulin
Protein Target Info & Infographic
Gene/Protein Information For NRG1 (Source: Uniprot.org, NCBI)
Gene Name
NRG1
Full Name
Pro-neuregulin-1, membrane-bound isoform
Weight
70.392kDa
Superfamily
neuregulin family
Alternative Names
NRG1 Beta 1, Heregulin-β1 NRG1 ARIA, GGF, GGF2, HGL, HRG, HRG1, HRGA, MST131, MSTP131, NDF-IT2, SMDF, NRG1 neuregulin 1 pro-neuregulin-1, membrane-bound isoform|acetylcholine receptor-inducing activity|glial growth factor 2|heregulin, alpha (45kD, ERBB2 p185-activator)|neu differentiation factor|pro-NRG1|sensory and motor neuron derived factor
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on NRG1, check out the NRG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NRG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human recombinant Heregulin Beta 1 protein, AF (PROTQ02297-3)
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