Human GDNF / Glial Derived Neurotrophic Factor PicoKine® Quick ELISA Kit

GDNF ELISA kit, 120-minute protocol

Human GDNF / Glial Derived Neurotrophic Factor PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human GDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml.

Product Info Summary

SKU: FEK0362
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 120 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human GDNF / Glial Derived Neurotrophic Factor PicoKine® Quick ELISA Kit

See all GDNF products

SKU/Catalog Number

FEK0362

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human GDNF / Glial Derived Neurotrophic Factor PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human GDNF in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human GDNF / Glial Derived Neurotrophic Factor PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0362)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: R109-I211

Sensitivity

<4 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0362 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0362 is reactive to GDNF in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0362 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of GDNF

Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.

Kit Components

Catalog number Description Quantity
FEK0362-CAP 96-well plate precoated with anti-Human GDNF antibody 1
EK0362-ST lyophilized recombinant Human GDNF standard 10 ng/tube
FEK0362-DA Biotinylated anti-Human GDNF detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0130.1140.1700.3180.6351.0251.6892.185

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)6926485566242810
Standard deviation4.9617.1636.76616.4537.26
CV (%)7.2%6.5%4.3%9.1%6.8%4.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GDNF in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 169767770733.534.8%
Sample 22642722672622663.761.4%
Sample 385594395281289059.076.6%
*number of samples for each test n=16.

Gene/Protein Information For GDNF (Source: Uniprot.Org, NCBI)

Uniprot ID

P39905

Gene ID

2668

Gene Name

GDNF

Full Name

Glial cell line-derived neurotrophic factor

Weight

23.72kDa

Superfamily

TGF-beta family

Alternative Names

Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3 GDNF ATF, ATF1, ATF2, HFB1-GDNF, HSCR3 glial cell derived neurotrophic factor glial cell line-derived neurotrophic factor|astrocyte-derived trophic factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GDNF, check out the GDNF Infographic

GDNF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GDNF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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1 Customer Q&As for Human GDNF / Glial Derived Neurotrophic Factor PicoKine® Quick ELISA Kit

Question

Just an inquiry about your product FEK0362, can the phenol red within the cell medium interfere with the measurement at 450nm? Can I use cellular supernatant without any pre-treatment?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

The phenol red should not interfere the measurement at 450nm, however we would suggest running a media control which acts as your negative control. About sample preparations no additional pre-treatment is necessary when using cell supernatant. Also acidifying the samples can enhance the activation of GDNF, so if you want to do this just don

Boster Scientific Support

Answered: 2020-04-17

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