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Product Info Summary
| SKU: | EZ1316 |
|---|---|
| Size: | For 5 plates, 96 wells each |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
Human CRP ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See all C-Reactive Protein/CRP products
SKU/Catalog Number
EZ1316
Size
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Description
Human CRP ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CRP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CRP ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1316)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: F17-P224
Assay Range
312 pg/ml - 20,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ1316 is reactive to CRP in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EZ1316 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog number | Description | Quantity | Storage of opened/reconstituted material |
|---|---|---|---|
| EZ1316-CA | Rabbit anti- human CRP polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
| EZ1316-DA | Biotinylated goat anti- human CRP polyclonal antibody (Detection Antibody) | 500 µl, 3.2 µg/mL (recommended dilution 1:100) | |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
| EK1316-ST | Lyophilized recombinant human CRP standard | 20 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.005 | 0.046 | 0.1 | 0.174 | 0.448 | 0.83 | 1.374 | 1.959 |
Data Example Images
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Human CRP EZ-Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 593 | 1488 | 8142 | 565 | 1551 | 8792 |
| Standard deviation | 36.17 | 62.49 | 643.21 | 38.42 | 75.99 | 861.61 |
| CV (%) | 6.1% | 4.2% | 7.9% | 6.8% | 4.9% | 9.8% |
Protein Target Info & Infographic
Gene/Protein Information For CRP (Source: Uniprot.Org, NCBI)
Gene Name
CRP
Full Name
C-reactive protein
Weight
25.039kDa
Superfamily
pentraxin family
Alternative Names
C reactive protein pentraxin related; C-reactive protein; C-reactive protein (1-205); CRP; CRP_HUMAN; MGC149895; MGC88244; Pentraxin 1; Pentraxin 1 short; PTX 1; PTX1; CRP PTX1 C-reactive protein C-reactive protein|C-reactive protein, pentraxin-related|pentraxin 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CRP, check out the CRP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CRP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CRP ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ1316)
Hello CJ!
EZ1316 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Efficacy of wIRA in the treatment of sacroiliitis in male patients with ankylosing spondylitis and its effect on serum VEGF levels
Effect of rosiglitazone on inflammatory cytokines and oxidative stress after intensive insulin therapy in patients with newly diagnosed type 2 diabetes
Effects of exercise therapy on knee joint function and synovial fluid cytokine levels in patients with knee osteoarthritis
Practical High-Performance Lateral Flow Assay Based on Autonomous Microfluidic Replacement on a Film
Elevated serum levels of soluble Axl in acute coronary syndrome
Asgary S, Keshvari M, Sahebkar A, Hashemi M, Rafieian-Kopaei M. Arya Atheroscler. 2013 Nov;9(6):326-31. Clinical Investigation Of The Acute Effects Of Pomegranate Juice On Blood Pressure And Endothelial Function In Hypertensive Individuals.
Iori E, Vinci B, Murphy E, Marescotti Mc, Avogaro A, Ahluwalia A. Plos One. 2012;7(4):E34704. Doi: 10.1371/Journal.Pone.0034704. Epub 2012 Apr 11. Glucose And Fatty Acid Metabolism In A 3 Tissue In-Vitro Model Challenged With Normo- And Hyperglyca...
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