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Product Info Summary
| SKU: | FEK1312 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human CD32 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1312
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Fc gamma RIIB/C (CD32b/c) (FCGR2B/C) PicoKine® Quick ELISA Kit (90 minues, 96 Tests). Quantitate Human FCGR2B/C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CD32 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1312)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A46-P217
Sensitivity
<10 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1312 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1312 is reactive to FCGR2B in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK1312 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCGR2B
CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1312-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1312-ST | Human FCGR2B Standard | 2 | 10 ng/tube |
| FEK1312-A | Human FCGR2B Antibody A | 1 | 3ml |
| FEK1312-B | Human FCGR2B Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.054 | 0.125 | 0.199 | 0.337 | 0.619 | 1.143 | 1.687 | 2.050 |
Data Example Images
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Human CD32/FCGR2b/c PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD32 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 122 | 404 | 1372 | 133 | 365 | 1478 |
| Standard deviation | 8.05 | 19.79 | 97.41 | 9.7 | 22.26 | 130.06 |
| CV (%) | 6.6% | 4.9% | 7.1% | 7.3% | 6.1% | 8.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FCGR2B in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 122 | 125 | 105 | 106 | 114 | 9.06 | 7.9% |
| Sample 2 | 404 | 361 | 357 | 357 | 369 | 19.84 | 5.3% |
| Sample 3 | 1372 | 1213 | 1353 | 1369 | 1326 | 66.06 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For FCGR2B (Source: Uniprot.Org, NCBI)
Gene Name
FCGR2B
Full Name
Low affinity immunoglobulin gamma Fc region receptor II-b
Weight
34.044kDa
Alternative Names
CD32 antigen; CD32b; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RIIB; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; FCGR2B; FcgRIIB; FCRIIB; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b FCGR2B CD32, CD32B, FCG2, FCGR2, FCGR2C, FcRII-c, IGFR2 Fc fragment of IgG receptor IIb low affinity immunoglobulin gamma Fc region receptor II-b|CDw32|Fc fragment of IgG, low affinity II, receptor for (CD32)|Fc fragment of IgG, low affinity IIb, receptor (CD32)|Fc fragment of IgG, low affinity IIb, receptor for (CD32)|Fc gamma RIIb|Fc gamma receptor IIb|Fc-gamma RII-c|Fc-gamma-RIIc|IgG Fc receptor II-c|Low affinity immunoglobulin gamma Fc region receptor II-c|fc-gamma RII-b|fc-gamma-RIIb|fcRII-b|igG Fc receptor II-b
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FCGR2B, check out the FCGR2B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCGR2B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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