Human ANTXR2 ELISA Kit PicoKine®

CMG-2/ANTXR2 ELISA kit for Human

Human ANTXR2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANTXR2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK2108
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human ANTXR2 ELISA Kit PicoKine®

View all CMG-2/ANTXR2 ELISA kits

SKU/Catalog Number

EK2108

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ANTXR2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ANTXR2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human ANTXR2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2108)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q34-G318

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2108 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2108 is reactive to Antxr2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2108 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CMG-2/ANTXR2

Anthrax toxin receptor 2 is a protein that in humans is encoded by the ANTXR2 gene. It is mapped to 4q21.21. This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Kit Components

Catalog Number Description Quantity
EK2108-CAP Anti-Human Antxr2 Pre-coated 96-well strip microplate 1
EK2108-ST Human Antxr2 Standard 2 vials, 10 ng/tube
EK2108-DA Human Antxr2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010,000
O.D.0.0000.0750.1730.2870.5250.9411.5422.314

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CMG-2/ANTXR2, Dilution ratio of 1:1, concentration in serum and plasma is around 3 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ANTXR2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)268970339330910284064
Standard deviation10.9941.71156.0817.9270.93292.61
CV (%)4.1%4.3%4.6%5.8%6.9%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CMG-2/ANTXR2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 118919021620119910.885.4%
Sample 216301733155315671620714.3%
Sample 351385161473749885006168.913.3%
*number of samples for each test n=16.

Gene/Protein Information For Antxr2 (Source: Uniprot.Org, NCBI)

Gene Name

Antxr2

Full Name

Anthrax toxin receptor 2

Weight

53.184kDa

Superfamily

ATR family

Alternative Names

anthrax toxin receptor 2; ANTXR2; Capillary morphogenesis gene 2 protein; capillary morphogenesis protein 2; cI-35; CMG2; CMG-2; CMG2MGC111533; CMG-2MGC45856; FLJ31074; ISH; JHF; JHS Antxr2|2310046B19Rik, AW561899, CMG-2, CMG2, cI-3, cI-35|anthrax toxin receptor 2|anthrax toxin receptor 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Antxr2, check out the Antxr2 Infographic

Antxr2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Antxr2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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6 Customer Q&As for Human ANTXR2 ELISA Kit PicoKine®

Question

Q: if the enzyme conjugated CMG-2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CMG-2 antigen?

Verified Customer

Verified customer

Asked: 2019-05-01

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-05-01

Question

Q: Can CMG-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-04-02

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-04-02

Question

Q: which procedure should I follow in order to thaw whole blood sample for CMG-2 ELISA after freezing?

W. Green

Verified customer

Asked: 2018-09-09

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CMG-2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-09-09

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

R. Wright

Verified customer

Asked: 2018-04-17

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-04-17

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-05-01

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-05-01

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CMG-2 in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

K. Mehta

Verified customer

Asked: 2016-03-27

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CMG-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2016-03-27

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