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Product Info Summary
| SKU: | EK7114 |
|---|---|
| Size: | 96 wells/kit |
| Reactive Species: | All species |
| Application: | ELISA |
| Sample Types: | Urine, Cell Lysates, Plasma, Sample matrices |
Product info
Product Name
DNA Damage (8-OHdG) ELISA Kit
See all DNA Damage (8-OHdG) products
SKU/Catalog Number
EK7114
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Colorimetric detection of DNA Damage (8-OHdG). 96 wells/kit.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
DNA Damage (8-OHdG) ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7114)
Clonality of Antibodies
See Datasheet for details
Sensitivity
0.59 ng/mL
Assay Range
0.94 - 60 ng/mL
Cross-Reactivity
8-Hydroxy-2-deoxy Guanosine (8-OHdG): 100%. 8-Hydroxy Guanosine (8-OHG): 23%. 8-Hydroxy Guanine (8-oxoG): 23%. Guanosine: <0.01%.
Reactive Species
EK7114 is reactive to DNA Damage (8-OHdG) in All species samples
Validated Sample Types
Urine, Cell Lysates, Plasma, Sample matrices
Application Guarantee
EK7114 is guaranteed for ELISA in All species by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Description | Quantity |
| 8-hydroxy-2-deoxy Guanosine : BSA Coated Plate | 1 Plate |
| 8-hydroxy-2-deoxy Guanosine Standard | 1 vial/ 100uL |
| 8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody | 1 vial/75uL |
| Sample and Standard Diluent | 1 vial/50mL |
| 8-hydroxy-2-deoxy Guanosine Antibody Diluent | 1 vial/13mL |
| Wash Buffer Concentrate | 1 vial/50mL |
| TMB Substrate | 1 vial/ 13mL |
| Stop Solution | 1 vial/ 13mL |
| Plate Cover | 2 covers |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Diagram of the Triplicate Sample Plate Format
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Diagram of the Preparation of the 8-OHdG Standards
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Urine Spike Assay
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Diagram of the 8-OHdG Competitive ELISA
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Chemical Equation of the Oxidation of Guanosine
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Typical Standard Curve for the DNA Damage (8-OHdG) ELISA Kit (Enzyme-Linked Immunosorbent Assay)K7114
Assay Type: Competitive ELISA.
Detection Method: Colorimetric Assay.
Assay Range: 0.94 60 ng/ml.
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DNA Damage (8-OHdG) in ELISA kits from four different production batches/lots.
Precision
Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.
Specific Publications For DNA Damage (8-OHdG) ELISA Kit (EK7114)
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Customer Q&As
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2 Customer Q&As for DNA Damage (8-OHdG) ELISA Kit
Question
What is the storage condition of EK7114 kit, fridge or deep freeze?
Verified customer
Asked: 2020-06-18
Answer
For the DNA Damage (8-OHdG) ELISA Kit (EK7114), all kit components are stable being stored at 4°C, with one exception. Kit component 8-hydroxy-2-deoxy Guanosine Standard must be kept at -20°C. For optimum storage, the 8-OHdG Standard should be aliquoted into smaller portions and then stored appropriately to reduce freeze/thaw cycles.
Boster Scientific Support
Answered: 2020-06-19
Question
Is the standard packed together with other components or is it in separate box when shipping the EK7114 kit?
Verified customer
Asked: 2020-06-18
Answer
The DNA Damage (8-OHdG) ELISA Kit (EK7114) components is shipped in bags – standard included with all other kit components.
Boster Scientific Support
Answered: 2020-06-18
