DNA Damage (8-OHdG) ELISA Kit

ELISA kit

Colorimetric detection of DNA Damage (8-OHdG). 96 wells/kit.

Product Info Summary

SKU: EK7114
Size: 96 wells/kit
Reactive Species: All species
Application: ELISA
Sample Types: Urine, Cell Lysates, Plasma, Sample matrices

Product Name

DNA Damage (8-OHdG) ELISA Kit

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SKU/Catalog Number

EK7114

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Colorimetric detection of DNA Damage (8-OHdG). 96 wells/kit.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

DNA Damage (8-OHdG) ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7114)

Clonality of Antibodies

See Datasheet for details

Sensitivity

0.59 ng/mL

Assay Range

0.94 - 60 ng/mL

Cross-Reactivity

8-Hydroxy-2-deoxy Guanosine (8-OHdG): 100%. 8-Hydroxy Guanosine (8-OHG): 23%. 8-Hydroxy Guanine (8-oxoG): 23%. Guanosine: <0.01%.

Reactive Species

EK7114 is reactive to in All species samples

Validated Sample Types

Urine, Cell Lysates, Plasma, Sample matrices

Application Guarantee

EK7114 is guaranteed for ELISA in All species by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

DescriptionQuantity
8-hydroxy-2-deoxy Guanosine : BSA Coated Plate1 Plate
8-hydroxy-2-deoxy Guanosine Standard1 vial/ 100uL
8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody1 vial/75uL
Sample and Standard Diluent1 vial/50mL
8-hydroxy-2-deoxy Guanosine Antibody Diluent1 vial/13mL
Wash Buffer Concentrate1 vial/50mL
TMB Substrate1 vial/ 13mL
Stop Solution1 vial/ 13mL
Plate Cover 2 covers

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Data Example Images

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of in ELISA kits from four different production batches/lots.

Precision

Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.

Gene/Protein Information For (Source: Uniprot.org, NCBI)

Gene Name

Full Name

Weight

Alternative Names

8-OH-dG; 8OHG; 80G; 8 hydroxyguanine; 8-OHdG; DNA Damage

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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2 Customer Q&As for DNA Damage (8-OHdG) ELISA Kit

Question

What is the storage condition of EK7114 kit, fridge or deep freeze?

Verified customer

Asked: 2020-06-18

Answer

For the DNA Damage (8-OHdG) ELISA Kit (EK7114), all kit components are stable being stored at 4°C, with one exception. Kit component 8-hydroxy-2-deoxy Guanosine Standard must be kept at -20°C. For optimum storage, the 8-OHdG Standard should be aliquoted into smaller portions and then stored appropriately to reduce freeze/thaw cycles.

Boster Scientific Support

Answered: 2020-06-19

Question

Is the standard packed together with other components or is it in separate box when shipping the EK7114 kit?

Verified customer

Asked: 2020-06-18

Answer

The DNA Damage (8-OHdG) ELISA Kit (EK7114) components is shipped in bags – standard included with all other kit components.

Boster Scientific Support

Answered: 2020-06-18

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