This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Tyrosine-protein kinase FRK.
Please shorten your article and try again.
Human | |
---|---|
Gene Name: | FRK |
Uniprot: | P42685 |
Entrez: | 2444 |
Belongs to: |
---|
protein kinase superfamily |
EC 2.7.10; Frk; fyn-related kinaseProtein-tyrosine kinase 5; Gtk; GTKEC 2.7.10.2; Nuclear tyrosine protein kinase RAK; PTK5 protein tyrosine kinase 5; PTK5; Rak; RAKPTK5; tyrosine-protein kinase FRK
Mass (kDA):
58.254 kDA
Human | |
---|---|
Location: | 6q22.1 |
Sequence: | 6; NC_000006.12 (115931149..116100739, complement) |
Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon.
Cytoplasm. Nucleus. Predominantly found in the nucleus, with a small fraction found in the cell periphery.
There are a few different uses for the FRK Marker, including Enhanced Chemiluminescence Detection, Colorimetric detection, and Autoradiography film. Learn more about the FRK Marker and how it can be used to perform these three types tests. Here are a few examples:
The FRK Marker (vector-path-based) is a marker. Its properties include its name, label, and color. The MarkerOptions can be used to modify its properties. The properties of a marker can be defined by the MarkerOptions class. It is only available in the beta channel. Its labelOrigin properties determine the label's placement. The marker's coord defines the non-transparent region where the marker can be clicked.
There are many uses for the FRK marker, but the most important is to maintain seam cells and then to divide them during larval development. The following are some examples:
Enhanced Chemiluminescence, also known as CL, is a popular method of detecting biological molecules. Its high sensitivity and wide dynamic range make it a favorite choice for quantitative biological analyses. It can be performed in a variety of settings including molecular biology, biochemistry, and cell biology. This article will cover the fundamental concept and practical applications that Enhanced Chemiluminescence is (CL).
The enhanced Chemiluminescence detection technique (CL), measures protein concentrations. The FRK indicator binds to an acridinium ring which reacts with the protein to create light. The interaction of an acridinium-phenol ring produces the signal. The FRK marker can be released by hydrolysis of the ester-bonded acridinium ring to the phenol.
To detect SEB, anti-SEB primary antibodies were immobilized on CNT surfaces. Then the mixture was placed on polycarbonate. The resulting FRK sign was measured by counting the number photons generated per second. Optical imaging of FRK markers has the potential of increasing the sensitivity of immunoassays. The researchers applied this technology to a food sample containing SEB. They placed anti-SEB prima antibodies on a CNT and then placed the antibody/nanotube mix on the polycarbonate.
The researchers used plasmonic nanparticles to enhance HRP chemical luminescence. The surface interactions with biotin-avidin induced HRP-avidin to be immobilized, while FRK streptavidin was induced by SIFs. The target protein could be detected at 6 nm away from the plasmonic particles.
A new colorimetric method combines reverse-blot hybridization and dynamic chemistry for the determination of Leishmania minor from Trypanosoma cruzi. This novel method uses a single-plex PCR step to amplify a homologous DNA sequence, which encodes a component of the large ribosome. The enzyme content in each well affects the intensity of the final product. This method is useful for kinetic studies and rapid, noninvasive diagnosis of the disease.
This method is much more sensitive than real-timePCR. However, the background signal is increased over long periods of incubation, obscuring the signal from target protein. This means that the detection limit of this technique is almost equal to real time PCR. However, only a few viral particles can be detected using the method. Using the new colorimetric biosensor based on AuNP interaction, the detection limit is significantly lower than that of real-time PCR.
Rapid MBD-based, colorimetric and electrochemical methods have successfully demonstrated DNAmethylation detection in clinical samples. These assays require very little sample and can be performed quickly, making them suitable for a wide variety of clinical applications. These methods yield results similar to those obtained with traditional PCR, but they don't require specialized equipment. However, the quality of the final images will affect the sensitivity of this technique.
The development and use of nanotechnology has opened up new possibilities for tumor markers. For colorimetric testing, nanomaterials have been used. They have been proven to be useful in the detection of cancer markers. They are particularly useful in cancer diagnosis. Further, they have potential for rapid and reliable diagnosis of cancer. The use of nanotechnology allows tumor markers to be detected more quickly than ever before.
The FRK proteins encode a polypeptide-chain that is highly specific to cells' cytoplasmic ends. Boster Bio is a manufacturer of antibodies and ELISA products since 1993. Boster Bio expanded its product offering to include PCR products related to molecular biology. Boster Bio provides a range services to scientists, including 24 hour technical support and technical resources for no charge.
Boster Bio provides high-affinity primary antibodies that have been validated on Western Blotting, Immunohistochemistry, and ELISA. FRK antibody reagents are available to use in a variety of applications, including immunofluorescence, ELISA, and a variety of molecular biology tests. Boster also offers blocking peptides that are available for various immunogen lengths.
PMID: 7696183 by Cance W.G., et al. Rak, a novel nuclear tyrosine kinase expressed in epithelial cells.
PMID: 7510261 by Lee J., et al. Cloning of FRK, a novel human intracellular SRC-like tyrosine kinase- encoding gene.