Product Info Summary
SKU: | A14735 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ZSCAN22/Hkr2 Antibody
SKU/Catalog Number
A14735
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ZSCAN22/Hkr2 Antibody (Catalog # A14735). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZSCAN22/Hkr2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14735)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ZSCAN22.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14735 is reactive to ZSCAN22 in Human
Reconstitution
Calculated molecular weight
54561 MW
Background of HKR2
May be involved in transcriptional regulation.
Ota T., Nat. Genet. 36:40-45(2004).
Bechtel S., BMC Genomics 8:399-399(2007).
Lichter P., Genomics 13:999-1007(1992).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14735 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells, using ZSCAN22 antibody A14735.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ZSCAN22 antibody A14735.
Protein Target Info & Infographic
Gene/Protein Information For ZSCAN22 (Source: Uniprot.org, NCBI)
Gene Name
ZSCAN22
Full Name
Zinc finger and SCAN domain-containing protein 22
Weight
54561 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Zinc finger and SCAN domain-containing protein 22;Krueppel-related zinc finger protein 2;Protein HKR2;Zinc finger protein 50;ZSCAN22;HKR2, ZNF50; ZSCAN22 HKR2, ZNF50 zinc finger and SCAN domain containing 22 zinc finger and SCAN domain-containing protein 22|GLI-Kruppel family member HKR2|krueppel-related zinc finger protein 2|zinc finger protein 50
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZSCAN22, check out the ZSCAN22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZSCAN22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZSCAN22/Hkr2 Antibody (A14735)
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No publications found for A14735
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Customer Q&As
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5 Customer Q&As for Anti-ZSCAN22/Hkr2 Antibody
Question
We are currently using anti-ZSCAN22/Hkr2 antibody A14735 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The anti-ZSCAN22/Hkr2 antibody (A14735) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-04
Question
I have a question about product A14735, anti-ZSCAN22/Hkr2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14735 anti-ZSCAN22/Hkr2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-09
Question
I see that the anti-ZSCAN22/Hkr2 antibody A14735 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-06
Question
I am interested in to test anti-ZSCAN22/Hkr2 antibody A14735 on human placenta for research purposes, then I may be interested in using anti-ZSCAN22/Hkr2 antibody A14735 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Carter
Verified customer
Asked: 2017-04-06
Answer
The products we sell, including anti-ZSCAN22/Hkr2 antibody A14735, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-04-06
Question
I have attached the WB image, lot number and protocol we used for placenta using anti-ZSCAN22/Hkr2 antibody A14735. Please let me know if you require anything else.
Z. Kulkarni
Verified customer
Asked: 2016-03-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-03-15