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Product Info Summary
| SKU: | M11505 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ZNRF2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M11505
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ZNRF2 Rabbit Monoclonal Antibody catalog # M11505. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZNRF2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11505)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IGB-26
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ZNRF2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M11505 is reactive to ZNRF2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
29 kDa
Calculated molecular weight
24115 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11505 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:100
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of ZNRF2 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ZNRF2 (Source: Uniprot.org, NCBI)
Gene Name
ZNRF2
Full Name
E3 ubiquitin-protein ligase ZNRF2
Weight
24115 MW
Alternative Names
E3 ubiquitin-protein ligase ZNRF2;6.3.2.-;Protein Ells2;RING finger protein 202;Zinc/RING finger protein 2;ZNRF2;RNF202; ZNRF2 RNF202 zinc and ring finger 2 E3 ubiquitin-protein ligase ZNRF2|RING finger protein 202|RING-type E3 ubiquitin transferase ZNRF2|protein Ells2|zinc and ring finger 2, E3 ubiquitin protein ligase|zinc finger/RING finger 2|zinc/RING finger protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZNRF2, check out the ZNRF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZNRF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZNRF2 Rabbit Monoclonal Antibody (M11505)
Hello CJ!
M11505 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xin X,Yao D,Zhang K,Han S,Liu D,Wang H,Liu X,Li G,Huang J,Wang J.Protective effects of Rosavin on bleomycin-induced pulmonary fibrosis via suppressing fibrotic and inflammatory signaling pathways in mice.Biomed Pharmacother.2019 Jul;115:108870.doi:10.1016/j.biopha.2019.108870.Epub 2019 Apr 23.PMID:31026730.
Species: Mouse
M11505 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:400
Qi Xiong,Xiang Tian,Weiling Li,Lin Chen,Mei Zhou,Congyue Xu,Qin Ru Sulforaphane alleviates methamphetamine-induced oxidative damage and apoptosis via the Nrf2-mediated pathway in vitro and in vivo.Food and Agricultural Immunology31(1)2020.https://doi.org
Species: Rat
M11505 usage in article: APP:WB, SAMPLE:PC12 CELLS, DILUTION:1:500
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-ZNRF2 Rabbit Monoclonal Antibody
Question
I am interested in to test anti-ZNRF2 Rabbit Monoclonal antibody M11505 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-ZNRF2 Rabbit Monoclonal antibody M11505 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-09-20
Answer
The products we sell, including anti-ZNRF2 Rabbit Monoclonal antibody M11505, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-20
Question
Can you help my question with product M11505, anti-ZNRF2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-31
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M11505 anti-ZNRF2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-31
Question
Does anti-ZNRF2 Rabbit Monoclonal antibody M11505 work for WB with cervix carcinoma erythroleukemia?
B. Yang
Verified customer
Asked: 2018-08-16
Answer
According to the expression profile of cervix carcinoma erythroleukemia, ZNRF2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-ZNRF2 Rabbit Monoclonal antibody M11505 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2018-08-16
Question
We are currently using anti-ZNRF2 Rabbit Monoclonal antibody M11505 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-15
Answer
The anti-ZNRF2 Rabbit Monoclonal antibody (M11505) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-15
Question
I was wanting to use your anti-ZNRF2 Rabbit Monoclonal antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
J. Gonzalez
Verified customer
Asked: 2014-07-03
Answer
It shows on the product datasheet, M11505 anti-ZNRF2 Rabbit Monoclonal antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-03
Question
Do you have a BSA free version of anti-ZNRF2 Rabbit Monoclonal antibody M11505 available?
K. Yang
Verified customer
Asked: 2013-07-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ZNRF2 Rabbit Monoclonal antibody M11505 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-07-29
