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Product Info Summary
| SKU: | A04894-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-WDR77 Antibody Picoband®
SKU/Catalog Number
A04894-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-WDR77 Antibody Picoband® catalog # A04894-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-WDR77 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04894-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human WDR77 recombinant protein (Position: A13-A295).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04894-1 is reactive to WDR77 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
56903 MW
Background of Wdr77
Methylosome protein 50 is a protein that in humans is encoded by the WDR77 gene. The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04894-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human HEK293 whole cell, human Hela whole cell, human U20S whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of WDR77 using anti-WDR77 antibody (A04894-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR77 antigen affinity purified polyclonal antibody (Catalog # A04894-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR77 at approximately 42 kDa. The expected band size for WDR77 is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For WDR77 (Source: Uniprot.org, NCBI)
Gene Name
WDR77
Full Name
Methylosome protein 50
Weight
56903 MW
Alternative Names
Vesicular acetylcholine transporter;VAChT;Solute carrier family 18 member 3;SLC18A3;VACHT; Wdr77|2610003I18Rik, 2610312E17Rik, C79984, p44/MEP, p44/MEP50|WD repeat domain 77|methylosome protein 50|MEP-50|WD repeat-containing protein 77
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WDR77, check out the WDR77 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WDR77: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WDR77 Antibody Picoband® (A04894-1)
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