Product Info Summary
SKU: | M10788 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-WASP Rabbit Monoclonal Antibody
SKU/Catalog Number
M10788
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-WASP Rabbit Monoclonal Antibody catalog # M10788. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-WASP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10788)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
23W45
Isotype
IgG
Immunogen
A synthesized peptide derived from human WASP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M10788 is reactive to WAS in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
60 kDa
Calculated molecular weight
70581 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M10788 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:40
FC 1:50
Positive Control
WB: human Jurkat whole cell, human HEL whole cell, human K562 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of WASP using anti-WASP antibody (M10788).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WASP antigen affinity purified monoclonal antibody (Catalog # M10788) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WASP at approximately 60 kDa. The expected band size for WASP is at 53 kDa.
Protein Target Info & Infographic
Gene/Protein Information For WAS (Source: Uniprot.org, NCBI)
Gene Name
WAS
Full Name
Wiskott-Aldrich syndrome protein
Weight
70581 MW
Alternative Names
5-aminolevulinate synthase, nonspecific, mitochondrial;ALAS-H;2.3.1.37;5-aminolevulinic acid synthase 1;Delta-ALA synthase 1;Delta-aminolevulinate synthase 1;ALAS1;ALAS3, ALASH;OK/SW-cl.121; WAS IMD2, SCNX, THC, THC1P, WASPA, WAS WASP actin nucleation promoting factor wiskott-Aldrich syndrome protein|eczema-thrombocytopenia|thrombocytopenia 1 (X-linked)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WAS, check out the WAS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WAS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WASP Rabbit Monoclonal Antibody (M10788)
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