Product Info Summary
SKU: | PB9869 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-VEGFD/FIGF Antibody Picoband®
SKU/Catalog Number
PB9869
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VEGFD/FIGF Antibody Picoband® catalog # PB9869. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGFD/FIGF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9869)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human VEGFD recombinant protein (Position: F89-R205). Human VEGFD shares 94% and 94.9% amino acid (aa) sequence identity with mouse and rat VEGFD, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9869 is reactive to VEGFD in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
40444 MW
Background of VEGFD
C-fos induced growth factor (FIGF) (or vascular endothelial growth factor D, VEGF-D) is a vascular endothelial growth factor that in humans is encoded by the FIGF gene. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. Analyzing by Northern blotting, Yamada et al. (1997) symbolized VEGFD, was expressed as a 2.2-kb transcript with highest expression in lung, heart, small intestine, and fetal lung, and lower levels in skeletal muscle, colon, and pancreas. And Achen et al. (1998) concluded that VEGFD was most closely related to VEGFC by virtue of the presence of N- and C-terminal extensions that were not found in other VEGF family members. Stacker et al. (2001) showed that VEGFD can induce tumor angiogenesis through VEGFR2 and tumor lymphangiogenesis through VEGFR3, whereas VEGF, which does not activate VEGFR3, induces only tumor angiogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9869 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat,
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Positive Control
WB: rat lung tissue, rat brain tissue, mouse lung tissue, mouse brain tissue
IHC: human colon cancer tissue, human lung cancer tissue, human lung cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of VEGFD using anti-VEGFD antibody (PB9869).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse lung tissue lysates,
Lane 4: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFD antigen affinity purified polyclonal antibody (Catalog # PB9869) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFD at approximately 40KD. The expected band size for VEGFD is at 40KD.
Click image to see more details
Figure 2. IHC analysis of VEGFD using anti-VEGFD antibody (PB9869).
VEGFD was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VEGFD Antibody (PB9869) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of VEGFD using anti-VEGFD antibody (PB9869).
VEGFD was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VEGFD Antibody (PB9869) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of VEGFD using anti-VEGFD antibody (PB9869).
VEGFD was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VEGFD Antibody (PB9869) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For VEGFD (Source: Uniprot.org, NCBI)
Gene Name
VEGFD
Full Name
Vascular endothelial growth factor D
Weight
40444 MW
Superfamily
PDGF/VEGF growth factor family
Alternative Names
Vascular endothelial growth factor D;VEGF-D;c-Fos-induced growth factor;FIGF;FIGF;VEGFD; VEGFD FIGF, VEGF-D vascular endothelial growth factor D vascular endothelial growth factor D|c-fos induced growth factor (vascular endothelial growth factor D)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VEGFD, check out the VEGFD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VEGFD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VEGFD/FIGF Antibody Picoband® (PB9869)
Hello CJ!
PB9869 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Efficacy and safety of 32P-nanocolloid for treatment of distant lymph node metastasis in VX2 tumor-bearing rabbits
The role of VEGF-C/D and Flt-4 in the lymphatic metastasis of early-stage invasive cervical carcinoma
VEGF-D-enhanced lymph node metastasis of ovarian cancer is reversed by vesicular stomatitis virus matrix protein
Du Lc, Chen Xc, Wang D, Wen Yj, Wang Ct, Wang Xm, Kan B, Wei Yq, Zhao X. Reprod Biol Endocrinol. 2014 Feb 6;12:14. Doi: 10.1186/1477-7827-12-14. Vegf-D-Induced Draining Lymphatic Enlargement And Tumor Lymphangiogenesis Promote Lymph Node Metastasi...
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Customer Q&As
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4 Customer Q&As for Anti-VEGFD/FIGF Antibody Picoband®
Question
My question regarding product PB9869, anti-VEGFD/FIGF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9869 anti-VEGFD/FIGF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-08
Question
My lab would like to test anti-VEGFD/FIGF antibody PB9869 on mouse lung for research purposes, then I may be interested in using anti-VEGFD/FIGF antibody PB9869 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The products we sell, including anti-VEGFD/FIGF antibody PB9869, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-06
Question
We are currently using anti-VEGFD/FIGF antibody PB9869 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
P. Jha
Verified customer
Asked: 2019-08-08
Answer
The anti-VEGFD/FIGF antibody (PB9869) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-08
Question
I see that the anti-VEGFD/FIGF antibody PB9869 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-20