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Product Info Summary
| SKU: | A16108 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-TTC39A Antibody Picoband®
SKU/Catalog Number
A16108
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TTC39A Antibody Picoband® catalog # A16108. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TTC39A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16108)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TTC39A recombinant protein (Position: E18-E489).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A16108 is reactive to TTC39A in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
24145 MW
Background of TTC39A
TTC39A (Tetratricopeptide Repeat Domain 39A) is located on human chromosome 5p13.3. The TTC39A protein is ubiquitously expressed in brain, colon and other tissues. The TTC39A (also known as DEME-6; C1orf34) gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. 232 organisms have orthologs with human gene TTC39A. An important paralog of this gene is TTC39B.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16108 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human MCF-7 whole cell, human 293T whole cell, human RT4 whole cell
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TTC39A using anti-TTC39A antibody (A16108).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC39A antigen affinity purified polyclonal antibody (Catalog # A16108) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC39A at approximately 72 kDa. The expected band size for TTC39A is at 70 kDa.
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Figure 2. Flow Cytometry analysis of 293T cells using anti-TTC39A antibody (A16108).
Overlay histogram showing 293T cells stained with A16108 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC39A Antibody (A16108, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For TTC39A (Source: Uniprot.org, NCBI)
Gene Name
TTC39A
Full Name
Tetratricopeptide repeat protein 39A
Weight
24145 MW
Superfamily
TTC39 family
Alternative Names
Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489 TTC39A C1orf34, DEME-6 tetratricopeptide repeat domain 39A tetratricopeptide repeat protein 39A|TPR repeat protein 39A|differentially expressed in MCF7 with estradiol protein 6|testicular tissue protein Li 213
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TTC39A, check out the TTC39A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TTC39A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TTC39A Antibody Picoband® (A16108)
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