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Product Info Summary
| SKU: | A08315-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-TSHZ1 Antibody Picoband®
View all Teashirt homolog 1 Antibodies
SKU/Catalog Number
A08315-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TSHZ1 Antibody Picoband® catalog # A08315-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TSHZ1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08315-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TSHZ1 recombinant protein (Position: K97-R800).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08315-1 is reactive to TSHZ1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
118 kDa
Calculated molecular weight
117.916kDa
Background of Teashirt homolog 1
Teashirt zinc finger homeobox 1 is a protein that in humans is encoded by the TSHZ1 gene. This gene encodes a colon cancer antigen that was defined by serological analysis of recombinant cDNA expression libraries. The encoded protein is a member of the teashirt C2H2-type zinc-finger protein family and may be involved in transcriptional regulation of developmental processes. Mutations in this gene may be associated with congenital aural atresia syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08315-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human RT4 whole cell, human 293T whole cell, human HL-60 whole cell, human Hela whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TSHZ1 using anti-TSHZ1 antibody (A08315-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSHZ1 antigen affinity purified polyclonal antibody (Catalog # A08315-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSHZ1 at approximately 118 kDa. The expected band size for TSHZ1 is at 118 kDa.
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Figure 2. IF analysis of TSHZ1 using anti-TSHZ1 antibody (A08315-1) and anti-Tubulin Alpha antibody (M03989-3).
TSHZ1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TSHZ1 Antibody (A08315-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For TSHZ1 (Source: Uniprot.org, NCBI)
Gene Name
TSHZ1
Full Name
Teashirt homolog 1
Weight
117.916kDa
Superfamily
teashirt C2H2-type zinc-finger protein family
Alternative Names
Histone-lysine N-methyltransferase SETDB2; Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2; SETDB2; C13orf4; CLLD8; KMT1F TSHZ1 CAA, NY-CO-33, SDCCAG33, TSH1 teashirt zinc finger homeobox 1 teashirt homolog 1| NY-CO-33|serologically defined colon cancer 33
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSHZ1, check out the TSHZ1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSHZ1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TSHZ1 Antibody Picoband® (A08315-1)
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