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Product Info Summary
| SKU: | M06819-18 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ChIP, ELISA, IHC, WB, Luminex |
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Product info
Product Name
Anti-Trimethyl-Histone H3 (Lys27) H3F3A Rabbit Monoclonal Antibody, Clone#RM175
SKU/Catalog Number
M06819-18
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Trimethyl-Histone H3 (Lys27) H3F3A Rabbit Monoclonal Antibody, Clone#RM175 (Catalog # M06819-18). Tested in ChIP, WB, IHC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Trimethyl-Histone H3 (Lys27) H3F3A Rabbit Monoclonal Antibody, Clone#RM175 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819-18)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM175
Isotype
Rabbit IgG
Immunogen
A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys27)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross-reactivity with monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.
Reactive Species
M06819-18 is reactive to H3F3A in Human, Vertebrates
Reconstitution
Calculated molecular weight
15328 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06819-18 is guaranteed for ChIP, ELISA, IHC, WB, Luminex Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ChIP: 1µg-5 µg
Western Blot: 1 ug/mL – 2 ug/mL
IHC: 0.5 ug/mL – 2 ug/mL
ELISA: 0.5ug/mL - 1 ug/mL
Luminex: 0.1 ug/mL – 0.5 ug/mL.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM175 at 1 ug/mL, showed a band of histone H3 trimethylated at Lysine 27 (K27me3) in HeLa cells.
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Figure 2. Specificity Test result
RM175 specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in Histone H3.
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Figure 3. IHC result
Immunohistochemistry staining of HepG2 cell using anti-Trimethyl-Histone H3 (Lys27) antibody, RM175.
Protein Target Info & Infographic
Gene/Protein Information For H3F3A (Source: Uniprot.org, NCBI)
Gene Name
H3F3A
Full Name
Histone H3.3
Weight
15328 MW
Superfamily
histone H3 family
Alternative Names
Histone H3.3;H3F3A;H3.3A, H3F3;PP781;H3F3B;H3.3B; H3-3A H3-3B, H3.3A, H3F3, H3F3A H3.3 histone A histone H3.3|H3 histone family member 3A|H3 histone, family 3A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H3F3A, check out the H3F3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H3F3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Trimethyl-Histone H3 (Lys27) H3F3A Rabbit Monoclonal Antibody, Clone#RM175 (M06819-18)
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