Product Info Summary
SKU: | A02049-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TIP49A/RUVBL1 Antibody Picoband®
SKU/Catalog Number
A02049-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TIP49A/RUVBL1 Antibody Picoband® catalog # A02049-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TIP49A/RUVBL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02049-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TIP49A/RUVBL1 recombinant protein (Position: Q13-D23).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02049-2 is reactive to RUVBL1 in Human, Monkey, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
54 kDa
Calculated molecular weight
37492 MW
Background of RUVBL1
RuvB-like 1 (E. coli), also known as RUVBL1 and TIP49, is a human gene. This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02049-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human K562 whole cell, human 293T whole cell, human HT1080 whole cell, monkey COS-7 whole cell, human MCF-7 whole cell, human Daudi whole cell, human MOLT-4 whole cell, human HEL whole cell, rat testis tissue, rat C6 whole cell, mouse testis tissue, mouse NIH/3T3 whole cell
IHC: human colorectal adenocarcinoma tissue, human thyroid cancer tissue, human lung adenocarcinoma tissue, human spleen tissue, human tonsil tissue, human ovarian cancer tissue
ICC/IF: A549 cell
IF: human colon cancer tissue
FCM: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HT1080 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
Lane 6: human Daudi whole cell lysates,
Lane 7: human MOLT-4 whole cell lysates,
Lane 8: human HEL whole cell lysates,
Lane 9: rat testis tissue lysates,
Lane 10: rat C6 whole cell lysates,
Lane 11: mouse testis tissue lysates,
Lane 12: mouse NIH/3T3 whole cell lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIP49A/RUVBL1 antigen affinity purified polyclonal antibody (Catalog # A02049-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIP49A/RUVBL1 at approximately 54 kDa. The expected band size for TIP49A/RUVBL1 is at 50 kDa.
Click image to see more details
Figure 2. IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2).
TIP49A/RUVBL1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2).
TIP49A/RUVBL1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2).
TIP49A/RUVBL1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2).
TIP49A/RUVBL1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2).
TIP49A/RUVBL1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2).
TIP49A/RUVBL1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 8. IF analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2).
TIP49A/RUVBL1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 9. IF analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2).
TIP49A/RUVBL1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 10. Flow Cytometry analysis of Hela cells using anti-TIP49A/RUVBL1 antibody (A02049-2).
Overlay histogram showing Hela cells stained with A02049-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIP49A/RUVBL1 Antibody (A02049-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RUVBL1 (Source: Uniprot.org, NCBI)
Gene Name
RUVBL1
Full Name
RuvB-like 1
Weight
37492 MW
Superfamily
RuvB family
Alternative Names
G2/mitotic-specific cyclin-B2; CCNB2 RUVBL1 ECP-54, ECP54, INO80H, NMP 238, NMP238, PONTIN, Pontin52, RVB1, TIH1, TIP49, TIP49A RuvB like AAA ATPase 1 ruvB-like 1|49 kDa TATA box-binding protein-interacting protein|49 kDa TBP-interacting protein|54 kDa erythrocyte cytosolic protein|INO80 complex subunit H|RuvB (E coli homolog)-like 1|RuvB-like AAA ATPase|TAP54-alpha|TATA binding protein interacting protein 49 kDa|TIP60-associated protein 54-alpha|epididymis secretory sperm binding protein|nuclear matrix protein 238|pontin 52
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RUVBL1, check out the RUVBL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RUVBL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIP49A/RUVBL1 Antibody Picoband® (A02049-2)
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