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Product Info Summary
| SKU: | A00019 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TGF beta 1 TGFB1 Antibody
SKU/Catalog Number
A00019
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-TGF beta 1 TGFB1 Antibody (Catalog # A00019). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-TGF beta 1 TGFB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00019)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
TGF beta 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of the mature growth factor (112 amino acids in length).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00019 is reactive to TGFB1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
44341 MW
Background of TGFB1
TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, including three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule; the amino acid sequence between human and mouse differs by only one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues, such as placenta and kidneys.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00019 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:20,000
IF Microscopy: User optimized
WB: 1:100 - 1:500
Validation Images & Assay Conditions
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Western blot analysis of TGF beta 1 in reduced recombinant TGF Beta 1 (lane 1), ladder (lane 2) and Non-reduced recombinant TGF Beta 1 (lane 3) using rabbit anti-TGF beta 1 affinity purified polyclonal antibody (Catalog # A00019) at 1:200. Load: 250 ng per lane. Predicted/Observed size: 12 kDa/25 kDa, 12 kDa for reduced TGF Beta 1/25 kDa for non-reduced TGF Beta 1. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Click image to see more details
ELISA results of rabbit anti-TGF beta 1 affinity purified polyclonal antibody (Catalog # A00019) tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1
Protein Target Info & Infographic
Gene/Protein Information For TGFB1 (Source: Uniprot.org, NCBI)
Gene Name
TGFB1
Full Name
Transforming growth factor beta-1 proprotein
Weight
44341 MW
Superfamily
TGF-beta family
Alternative Names
TGFB1, TGF beta 1, TGF ß 1, Transforming growth factor beta-1, Latency-associated peptide, LAP, TGFB 1 TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TGFB1, check out the TGFB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TGFB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TGF beta 1 TGFB1 Antibody (A00019)
Hello CJ!
A00019 has been cited in 25 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ge Y,Cheng R,Sun S,Zhang S,Li L,Jiang J,Yang C,Xuan X,Chen J.Fangxiao Formula alleviates airway inflammation and remodeling in rats with asthma via suppression of transforming growth factor -β/Smad3 signaling pathway.Biomed Pharmacother.2019 Nov;119:109429.doi:10.1016/j.biopha.2019.109429.Epub 2019 Sep 7.PMID:31505422.
Species: Rat
A00019 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:NA
Jiang Peng-Fei,Peng Jun,Tan Han-Yu,Ou-Yang Yun,Qin Gen-Yan,Peng Qing-Hua,Effects of Buddlejae Flos Granules on Inflammatory Factors TGF-β1, NF-κB, IL-10 and IL-12 in Lacrimal Gland Cells of Castrated Male Rabbits,Digital Chinese Medicine,Volume 2,Issue 2,2019,Pages 97-104,ISSN 2589-3777,https://doi.org/10.1016/j.dcmed.2019.09.004.
Species: New Zealand White Rabbit
A00019 usage in article: APP:IHC, SAMPLE:LACRIMAL GLAND CELL, DILUTION:NA
Yao Ming,Sijing Cheng,Wen Long,Hong-Lian Wang,Chuanlan Xu,Xiaoyu Liu,Qiong Zhang,Sha Zhao,Xia Zou,Junming Fan,Li Wang,Hongchun Shen,"The Herbal Formula Granule Prescription Mahuang Decoction Ameliorated Chronic Kidney Disease Which Was Associated with Restoration of Dysbiosis of Intestinal Microbiota in Rats",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 4602612,12 pages,2021.https://doi.org/10.1155/2021/4602612
Species: Rat
A00019 usage in article: APP:IHC, SAMPLE:KIDNEY TISSUE, DILUTION:1:100
Qu X,Fang Y,Zhuang S,Zhang Y.Micro-RNA miR-542-3p suppresses decidualization by targeting ILK pathways in human endometrial stromal cells.Sci Rep.2021 Mar 30;11(1):7186.doi:10.1038/s41598-021-85295-2.PMID:33785768;PMCID:PMC8009905.
Species: Human,Mouse
A00019 usage in article: APP:WB, SAMPLE:HESCS, DILUTION:1:200
Huanyue Cui,Xueying Liu,Jin Zhang,Ke Zhang,Dahong Yao,Shi Dong,Shushu Feng,Lu Yang, Yuyao Li,Hangyu Wang,Jian Huang,Jinhui Wang,"Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways",Evidence-Based Complementary and Alternative Medicine,vol. 2021,Article ID 6103158,18 pages,2021.https://doi.org/10.1155/2021/6103158
Species: Human,Rat
A00019 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:800
Xu,R.,Zhou,X., Li,Y.,Ren,L.,& He,X. (2021).MicroRNA‑181a promotes epithelial‑mesenchymal transition in esophageal squamous cell carcinoma via the TGF‑β/Smad pathway.Molecular Medicine Reports,23,316. https://doi.org/10.3892/mmr.2021.11955
Species: Human
A00019 usage in article: APP:WB, SAMPLE:TE-1 CELL, DILUTION:1:100-1:400
Nie L,Liu M,Chen J,Wu Q,Li Y,Yi J,Zheng X,Zhang J,Chu C,Yang J.Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.Mol Med Rep.2021 Apr;23(4):299.doi:10.3892/mmr.2021.11938.Epub 2021 Mar 2.PMID:336498
Species: Rat
A00019 usage in article: APP:WB, SAMPLE:VENTRICULAR TISSUE, DILUTION:1:500
Lian Y,Zhu M,Chen J,Yang B,Lv Q,Wang L,Guo S,Tan X,Li C,Bu W,Ding W,Jia X,Feng L. Characterization of a novel polysaccharide from Moutan Cortex and its ameliorative effect on AGEs-induced diabetic nephropathy. Int J Biol Macromol. 2021 Feb 10:S0141-8130(2
Species: Human,Rat
A00019 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:200
Zhang T,Shen Z,Zheng J,Jiang R.Effect of UVA1 on hypertrophic scarring in the rabbit ear model.Biosci Rep.2020 Jan 31;40(1):BSR20190007.doi:10.1042/BSR20190007. PMID:31894858;PMCID:PMC6974420.
Species: New Zealand Rabbit
A00019 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:1:100
Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498.
Species: Rat
A00019 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-TGF beta 1 TGFB1 Antibody
Question
My question regards to test anti-TGF beta 1 antibody A00019 on human leukocyte for research purposes, then I may be interested in using anti-TGF beta 1 antibody A00019 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The products we sell, including anti-TGF beta 1 antibody A00019, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-04
Question
I was wanting to use your anti-TGF beta 1 antibody for IF for human leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
It shows on the product datasheet, A00019 anti-TGF beta 1 antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-27
Question
We have seen staining in human plasma. Are there any suggestions? Is anti-TGF beta 1 antibody supposed to stain plasma positively?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
From literature plasma does express TGFB1. From Uniprot.org, TGFB1 is expressed in leukocyte, duodenum eye, carcinoma, urinary bladder carcinoma, plasma, platelet, among other tissues. Regarding which tissues have TGFB1 expression, here are a few articles citing expression in various tissues:
Duodenum, and Eye, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Urinary bladder carcinoma, Pubmed ID: 8471846
Boster Scientific Support
Answered: 2020-01-15
Question
I have a question about product A00019, anti-TGF beta 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00019 anti-TGF beta 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-25
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-TGF beta 1 antibody A00019. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
We bought anti-TGF beta 1 antibody for WB on leukocyte in the past. I am using human, and We intend to use the antibody for IF next. I was wanting to use examining leukocyte as well as urinary bladder carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
I took a look at the website and datasheets of our anti-TGF beta 1 antibody and I see that A00019 has been validated on human in both WB and IF. Thus A00019 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-19
Question
Do you have a BSA free version of anti-TGF beta 1 antibody A00019 available?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TGF beta 1 antibody A00019 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-03
Question
I see that the anti-TGF beta 1 antibody A00019 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-24
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-24
Question
We are currently using anti-TGF beta 1 antibody A00019 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
The anti-TGF beta 1 antibody (A00019) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-16
Question
I have attached the WB image, lot number and protocol we used for leukocyte using anti-TGF beta 1 antibody A00019. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-15
Question
Is this A00019 anti-TGF beta 1 antibody reactive to the isotypes of TGFB1?
Verified Customer
Verified customer
Asked: 2018-04-13
Answer
The immunogen of A00019 anti-TGF beta 1 antibody is TGF beta 1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of the mature growth factor (112 amino acids in length). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-13
Question
Is a blocking peptide available for product anti-TGF beta 1 antibody (A00019)?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
We do provide the blocking peptide for product anti-TGF beta 1 antibody (A00019). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-13
Question
Will anti-TGF beta 1 antibody A00019 work for IF with leukocyte?
R. Krishna
Verified customer
Asked: 2016-03-04
Answer
According to the expression profile of leukocyte, TGFB1 is highly expressed in leukocyte. So, it is likely that anti-TGF beta 1 antibody A00019 will work for IF with leukocyte.
Boster Scientific Support
Answered: 2016-03-04
Question
My team were well pleased with the WB result of your anti-TGF beta 1 antibody. However we have seen positive staining in plasma latency-associated peptide: secreted, using this antibody. Is that expected? Could you tell me where is TGFB1 supposed to be expressed?
V. Carter
Verified customer
Asked: 2014-05-07
Answer
Based on literature, plasma does express TGFB1. Generally TGFB1 expresses in latency-associated peptide: secreted,, transforming growth factor beta-1: secreted. Regarding which tissues have TGFB1 expression, here are a few articles citing expression in various tissues:
Duodenum, and Eye, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Urinary bladder carcinoma, Pubmed ID: 8471846
Boster Scientific Support
Answered: 2014-05-07
Question
Would A00019 anti-TGF beta 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Zhao
Verified customer
Asked: 2013-12-13
Answer
As indicated on the product datasheet, A00019 anti-TGF beta 1 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-12-13
