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Product Info Summary
| SKU: | A03075-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-REG1A/1B Antibody Picoband®
SKU/Catalog Number
A03075-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-REG1A/1B Antibody Picoband® catalog # A03075-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-REG1A/1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03075-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human REG1A/1B recombinant protein (Position: N51-E90).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03075-2 is reactive to REG1A/1B in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
19 kDa
Calculated molecular weight
49162 MW
Background of REG1A/1B
This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03075-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat small intestine tissue, mouse small intestine tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of REG1A/1B using anti-REG1A/1B antibody (A03075-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat small intestine tissue lysates,
Lane 2: mouse small intestine tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REG1A/1B antigen affinity purified polyclonal antibody (Catalog # A03075-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REG1A/1B at approximately 19 kDa. The expected band size for REG1A/1B is at 19 kDa.
Protein Target Info & Infographic
Gene/Protein Information For REG1A/1B (Source: Uniprot.org, NCBI)
Gene Name
REG1A/1B
Full Name
Weight
49162 MW
Alternative Names
Metastasis-associated protein MTA2; Metastasis-associated 1-like 1; MTA1-L1 protein; p53 target protein in deacetylase complex; MTA2; MTA1L1; PID
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on REG1A/1B, check out the REG1A/1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for REG1A/1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-REG1A/1B Antibody Picoband® (A03075-2)
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