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Product Info Summary
| SKU: | A03660-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-PIST/GOPC Antibody Picoband®
SKU/Catalog Number
A03660-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PIST/GOPC Antibody Picoband® catalog # A03660-2. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PIST/GOPC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03660-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PIST/GOPC recombinant protein (Position: M30-Y462).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03660-2 is reactive to GOPC in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
28461 MW
Background of PIST
Golgi-associated PDZ and coiled-coil motif-containing protein is a protein that in humans is encoded by the GOPC gene. This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03660-2 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human HEK293 whole cell, human PC-3 whole cell
IHC: human breast cancer tissue, human liver cancer tissue
ICC/IF: SiHa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PIST/GOPC using anti-PIST/GOPC antibody (A03660-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIST/GOPC antigen affinity purified polyclonal antibody (Catalog # A03660-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIST/GOPC at approximately 55 kDa. The expected band size for PIST/GOPC is at 55 kDa.
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Figure 2. IHC analysis of PIST/GOPC using anti-PIST/GOPC antibody (A03660-2).
PIST/GOPC was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIST/GOPC Antibody (A03660-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of PIST/GOPC using anti-PIST/GOPC antibody (A03660-2).
PIST/GOPC was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIST/GOPC Antibody (A03660-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IF analysis of PIST/GOPC using anti-PIST/GOPC antibody (A03660-2).
PIST/GOPC was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PIST/GOPC Antibody (A03660-2) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For GOPC (Source: Uniprot.org, NCBI)
Gene Name
GOPC
Full Name
Golgi-associated PDZ and coiled-coil motif-containing protein
Weight
28461 MW
Alternative Names
DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46; MCM5; CDC46 GOPC CAL, FIG1, PIST, dJ94G16.2, GOPC golgi associated PDZ and coiled-coil motif containing Golgi-associated PDZ and coiled-coil motif-containing protein|CFTR-associated ligand|PDZ protein interacting specifically with TC10|PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10|dJ94G16.2 PIST|fused in glioblastoma|golgi-associated PDZ and coiled-coil motif containing protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GOPC, check out the GOPC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GOPC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PIST/GOPC Antibody Picoband® (A03660-2)
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