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Product Info Summary
| SKU: | P00279-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Phospho-Merlin (Ser518) NF2 Antibody
View all NF2/Merlin Antibodies
SKU/Catalog Number
P00279-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Merlin (Ser518) NF2 Antibody (Catalog # P00279-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Merlin (Ser518) NF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00279-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around phosphorylation site of serine 518 (R-L-S(p)-M-E) derived from Human Merlin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P00279-1 is reactive to NF2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
69690 MW
Background of NF2/Merlin
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity.
Guang-Hui Xiao, et al. (2005) Mol. Cell. Biol ; 25: 2384 - 2394.
Hi-Su Yang, et al. (2006) Cancer Res ; 66: 2708 - 2715.
R Bohni, et al. (1994) J. Biol. Chem ; 269: 14541 - 14545.
Adam J. Ratner, et al. (2001) J. Biol. Chem ; 276: 19267 - 19275.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00279-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 69kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts from HUVEC cells untreated(lane 1) or treated with IFN-a(lane 2) using Merlin(Phospho-Ser518) antibody P00279-1.
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Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Merlin (Phospho-Ser518) antibody P00279-1.
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Immunofluorescence staining of methanol-fixed Hela cells using Merlin(Phospho-Ser518) antibody P00279-1.
Protein Target Info & Infographic
Gene/Protein Information For NF2 (Source: Uniprot.org, NCBI)
Gene Name
NF2
Full Name
Merlin
Weight
69690 MW
Alternative Names
Merlin;Moesin-ezrin-radixin-like protein;Neurofibromin-2;Schwannomerlin;Schwannomin;NF2;SCH; NF2 ACN, BANF, SCH, merlin-1 neurofibromin 2 merlin|bilateral acoustic neurofibromatosis|moesin-ezrin-radixin like|moesin-ezrin-radixin-like protein|moesin-ezrin-radizin-like protein|neurofibromin 2 (bilateral acoustic neuroma)|schwannomerlin|schwannomin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NF2, check out the NF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Merlin (Ser518) NF2 Antibody (P00279-1)
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No publications found for P00279-1
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