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Product Info Summary
| SKU: | A03275-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-PDIP/PDIA2 Antibody Picoband®
SKU/Catalog Number
A03275-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PDIP/PDIA2 Antibody Picoband® catalog # A03275-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PDIP/PDIA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03275-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PDIP/PDIA2 recombinant protein (Position: Y117-D492).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03275-1 is reactive to PDIA2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
58.206kDa
Background of PDI
Protein disulfide isomerase family A member 2 is a protein that in humans is encoded by the PDIA2 gene. This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. The protein plays a role in the folding of nascent proteins in the endoplasmic reticulum by forming disulfide bonds through its thiol isomerase, oxidase, and reductase activity. The encoded protein also possesses estradiol-binding activity and can modulate intracellular estradiol levels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03275-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human PCP tissue
FCM: 293T cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PDIP/PDIA2 using anti-PDIP/PDIA2 antibody (A03275-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PCP tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIP/PDIA2 antigen affinity purified polyclonal antibody (Catalog # A03275-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDIP/PDIA2 at approximately 65 kDa. The expected band size for PDIP/PDIA2 is at 68 kDa.
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Figure 2. Flow Cytometry analysis of 293T cells using anti-PDIP/PDIA2 antibody (A03275-1).
Overlay histogram showing 293T cells stained with A03275-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDIP/PDIA2 Antibody (A03275-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PDIA2 (Source: Uniprot.org, NCBI)
Gene Name
PDIA2
Full Name
Protein disulfide-isomerase A2
Weight
58.206kDa
Superfamily
protein disulfide isomerase family
Alternative Names
Leucine-rich PPR motif-containing protein, mitochondrial; 130 kDa leucine-rich protein; LRP 130; GP130; LRPPRC; LRP130 PDIA2 PDA2, PDI, PDIP, PDIR protein disulfide isomerase family A member 2 protein disulfide-isomerase A2|Rho GDP dissociation inhibitor gamma|pancreas-specific protein disulfide isomerase|pancreatic protein disulfide isomerase|protein disulfide isomerase, pancreatic|protein disulfide isomerase-associated 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDIA2, check out the PDIA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDIA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PDIP/PDIA2 Antibody Picoband® (A03275-1)
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