Anti-Parathyroid Hormone (PTH) (N-Terminal) Monoclonal Antibody

Product Name

Anti-Parathyroid Hormone (PTH) (N-Terminal) Monoclonal Antibody

View all PTH Antibodies

SKU/Catalog Number

M00208

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Parathyroid Hormone (PTH) (N-Terminal) Monoclonal Antibody (Catalog # M00208). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Parathyroid Hormone (PTH) (N-Terminal) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00208)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: 3H9

Isotype

IgG2b, kappa

Immunogen

A synthetic peptide around aa 1-34 of human mature-PTH-polypeptide (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00208 is reactive to PTH in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

12861 MW

Background of PTH

Epitope of This monoclonal antibody maps in between aa 1-34. PTH is a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion.It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00208 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For PTH (Source: Uniprot.org, NCBI)

Alternative Names

Parathyroid hormone;PTH;Parathormone;Parathyrin;PTH; PTH FIH11, PTH parathyroid hormone parathyroid hormone|parathormone|parathyrin|parathyroid hormone 1|prepro-PTH|preproparathyroid hormone

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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