Anti-PAPP A/PAPPA Antibody Picoband®

Product Name

Anti-PAPP A/PAPPA Antibody Picoband®

View all Pappalysin-1/PAPP-A Antibodies

SKU/Catalog Number

PB9306

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PAPP A/PAPPA Antibody Picoband® catalog # PB9306. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PAPP A/PAPPA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9306)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PAPP A recombinant protein (Position: R95-Q388). Human PAPP A shares 88% amino acid (aa) sequence identity with mouse PAPP A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9306 is reactive to PAPPA in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

181 kDa

Calculated molecular weight

180973 MW

Background of Pappalysin-1/PAPP-A

Pappalysin-1, also known as DIPLA1, is a protein that in humans is encoded by the PAPPA gene. It is mapped to 9q33.1. PAPPA is found in the ovarian follicles, follicular fluid, luteal cells, and fallopian tubes of nonpregnant women and in the seminal vesicles and seminal fluid of males. This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. It has been found that circulating PAPPA is a disulfide-bridged complex with proMBP in which the subunits of the constituents are present in a 1:1 molar ratio.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9306 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: Human Placenta Tissue, HT1080 Whole Cell, SKOV Whole Cell, 22RV1 Whole Cell, SW620 Whole Cell, MM231 Whole Cell
IHC: Human Placenta Tissue

Validation Images & Assay Conditions

Click image to see more details

Anti-PAPP A Picoband antibody, PB9306, Western blotting
All lanes: Anti PAPP A (PB9306) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: SKOV Whole Cell Lysate at 40ug
Lane 4: 22RV1 Whole Cell Lysate at 40ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Lane 6: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 181KD
Observed bind size: 181KD

Click image to see more details

Anti-PAPP A Picoband antibody, PB9306, IHC(P)
IHC(P): Human Placenta Tissue

Gene/Protein Information For PAPPA (Source: Uniprot.org, NCBI)

Alternative Names

Pappalysin-1;3.4.24.79;Insulin-like growth factor-dependent IGF-binding protein 4 protease;IGF-dependent IGFBP-4 protease;IGFBP-4ase;Pregnancy-associated plasma protein A;PAPP-A;PAPPA; PAPPA ASBABP2, DIPLA1, IGFBP-4ase, PAPA, PAPP-A1, PAPPA pappalysin 1 pappalysin-1|IGF-dependent IGFBP-4 protease|aspecific BCL2 ARE-binding protein 2|differentially placenta 1 expressed protein|insulin-like growth factor-dependent IGF binding protein-4 protease|pregnancy-associated plasma protein A, pappalysin 1

For more info on PAPPA, check out the PAPPA Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PAPPA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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