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Product Info Summary
| SKU: | M02930 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) ENO2 Monoclonal Antibody
View all Enolase 2/Neuron-specific Enolase Antibodies
SKU/Catalog Number
M02930
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) ENO2 Monoclonal Antibody (Catalog # M02930). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) ENO2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02930)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: ENO2/1375
Isotype
IgG2b, kappa
Immunogen
A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M02930 is reactive to ENO2 in Human, Mouse, Rat
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
47269 MW
Background of Enolase 2/Neuron-specific Enolase
Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02930 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (0.2-0.4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Pheochromocytoma stained with Anti-NSE gamma Monoclonal Antibody (ENO2/1375).
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Formalin-fixed, paraffin-embedded human Cerebellum stained with Anti-NSE gamma Monoclonal Antibody (ENO2/1375).
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Western Blot of Y79, HeLa and HepG2 cell lysate using Anti-NSE, gamma Monoclonal Antibody (ENO2/1375).
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Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Anti-NSE gamma Mouse Monoclonal Antibody (ENO2/1375).
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Formalin-fixed, paraffin-embedded Rat Heart stained with Anti-NSE gamma Mouse Monoclonal Antibody (ENO2/1375).
Protein Target Info & Infographic
Gene/Protein Information For ENO2 (Source: Uniprot.org, NCBI)
Gene Name
ENO2
Full Name
Gamma-enolase
Weight
47269 MW
Superfamily
enolase family
Alternative Names
Gamma-enolase;4.2.1.11;2-phospho-D-glycerate hydro-lyase;Enolase 2;Neural enolase;Neuron-specific enolase;NSE;ENO2; ENO2 HEL-S-279, NSE enolase 2 gamma-enolase|2-phospho-D-glycerate hydro-lyase|2-phospho-D-glycerate hydrolyase|enolase 2 (gamma, neuronal)|epididymis secretory protein Li 279|neural enolase|neuron specific gamma enolase|neuron-specific enolase|neuronal enriched enolase|neurone-specific enolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ENO2, check out the ENO2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ENO2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) ENO2 Monoclonal Antibody (M02930)
Hello CJ!
M02930 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
High glucose upregulates myosin light chain kinase to induce microfilament cytoskeleton rearrangement in hippocampal neurons
MicroRNA-24 alleviates isoflurane-induced neurotoxicity in rat hippocampus via attenuation of oxidative stress
Xu,Y.,Liu,S.,Zhu,L.,Dai,L.,Qian,W.,Zhang,J. ... Pan,W.(2021).Green tea protects against hippocampal neuronal apoptosis in diabetic encephalopathy by inhibiting JNK/MLCK signaling.Molecular Medicine Reports,24,575.https://doi.org/10.3892/mmr.2021.12214
Species: Rat
M02930 usage in article: APP:IF, SAMPLE:HIPPOCAMPAL NEURONS, DILUTION:1:100
Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells
Protective effects of Dendrobium nobile Lindl. alkaloids on amyloid beta (25%u201335)-induced neuronal injury
%u03B2-Elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells
Involvement of calmodulin and actin in directed differentiation of rat cortical neural stem cells into neurons
High glucose upregulates myosin light chain kinase to induce microfilament cytoskeleton rearrangement in hippocampal neurons
HIF-1? effects on angiogenic potential in human small cell lung carcinoma
Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by co-culture with retinal pigmented epithelial cells
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) ENO2 Monoclonal Antibody
Question
I see that the anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Monoclonal antibody M02930 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-29
Question
Do you have a BSA free version of anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Monoclonal antibody M02930 available?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Monoclonal antibody M02930 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-28
Question
Will M02930 anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
You can see on the product datasheet, M02930 anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-23
Question
Does anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Monoclonal antibody M02930 work for IHC with cervix carcinoma?
L. Bhatt
Verified customer
Asked: 2015-09-22
Answer
According to the expression profile of cervix carcinoma, ENO2 is highly expressed in cervix carcinoma. So, it is likely that anti-NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Monoclonal antibody M02930 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2015-09-22
