Product Info Summary
SKU: | A06319-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NOV/CCN3 Antibody Picoband®
SKU/Catalog Number
A06319-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NOV/CCN3 Antibody Picoband® catalog # A06319-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NOV/CCN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06319-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NOV/CCN3, different from the related mouse sequence by seven amino acids, and from the related rat sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06319-1 is reactive to NOV in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
39162 MW
Background of CCN3
NOV (nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06319-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell
IHC: human intestinal cancer tissue, human lung cancer tissue, human mammary cancer tissue, rat kidney tissue
ICC/IF: A431 cell
FCM: U87 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 2. IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (A06319-1).
NOV/CCN3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOV/CCN3 Antibody (A06319-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 1. Western blot analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (A06319-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOV/CCN3 antigen affinity purified polyclonal antibody (Catalog # A06319-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOV/CCN3 at approximately 47KD. The expected band size for NOV/CCN3 is at 39KD.
Click image to see more details
Figure 3. IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (A06319-1).
NOV/CCN3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOV/CCN3 Antibody (A06319-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (A06319-1).
NOV/CCN3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOV/CCN3 Antibody (A06319-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (A06319-1).
NOV/CCN3 was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NOV/CCN3 Antibody (A06319-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IF analysis of NOV/CCN3 using anti-NOV/CCN3 antibody (A06319-1).
NOV/CCN3 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NOV/CCN3 Antibody (A06319-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 7. Flow Cytometry analysis of U87 cells using anti-NOV/CCN3 antibody (A06319-1).
Overlay histogram showing U87 cells stained with A06319-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOV/CCN3 Antibody (A06319-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For NOV (Source: Uniprot.org, NCBI)
Gene Name
NOV
Full Name
CCN family member 3
Weight
39162 MW
Superfamily
CCN family
Alternative Names
Protein NOV homolog;NovH;CCN family member 3;Insulin-like growth factor-binding protein 9;IBP-9;IGF-binding protein 9;IGFBP-9;Nephroblastoma-overexpressed gene protein homolog;NOV;CCN3, IGFBP9, NOVH; CCN3 IBP-9, IGFBP-9, IGFBP9, NOV, NOVh cellular communication network factor 3 CCN family member 3|IGF-binding protein 9|insulin-like growth factor-binding protein 9|nephro blastoma-overexpressed gene protein homolog|nephroblastoma overexpressed|nephroblastoma-overexpressed gene protein homolog|protein NOV homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOV, check out the NOV Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOV: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOV/CCN3 Antibody Picoband® (A06319-1)
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Customer Q&As
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4 Customer Q&As for Anti-NOV/CCN3 Antibody Picoband®
Question
Would anti-NOV/CCN3 antibody A06319-1 work for IHC with left adrenal gland?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
According to the expression profile of left adrenal gland, NOV is highly expressed in left adrenal gland. So, it is likely that anti-NOV/CCN3 antibody A06319-1 will work for IHC with left adrenal gland.
Boster Scientific Support
Answered: 2020-02-21
Question
We are currently using anti-NOV/CCN3 antibody A06319-1 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The anti-NOV/CCN3 antibody (A06319-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-26
Question
I was wanting to use your anti-NOV/CCN3 antibody for IHC for rat left adrenal gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat left adrenal gland identification?
Verified Customer
Verified customer
Asked: 2019-04-02
Answer
It shows on the product datasheet, A06319-1 anti-NOV/CCN3 antibody has been tested for IHC, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat left adrenal gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-02
Question
Is there a BSA free version of anti-NOV/CCN3 antibody A06319-1 available?
Verified Customer
Verified customer
Asked: 2018-04-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NOV/CCN3 antibody A06319-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-13