Product Info Summary
SKU: | A04830-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-NOP2 Antibody Picoband®
SKU/Catalog Number
A04830-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NOP2 Antibody Picoband® catalog # A04830-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NOP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04830-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NOP2 recombinant protein (Position: L210-Q747).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04830-1 is reactive to NOP2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
120 kDa
Calculated molecular weight
89.302kDa
Background of NOL1
Enables RNA binding activity. Involved in positive regulation of cell population proliferation; regulation of signal transduction by p53 class mediator; and ribosomal large subunit assembly. Located in nucleolus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04830-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human HEL whole cell, human Caco-2 whole cell, human A549 whole cell, human Hela whole cell, human RT4 whole cell, human 293T tissue, human SIHA whole cell lyates, human U251 whole cell
ICC/IF: U2OS cell
FCM: RT4 cell
Validation Images & Assay Conditions
![a04830 1 nop2 primary antibodies wb testing 1 a04830 1 nop2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a04830-1-nop2-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of NOP2 using anti-NOP2 antibody (A04830-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human RT4 whole cell lysates,
Lane 6: human 293T tissue lysates,
Lane 7: human SIHA whole cell lyates,
Lane 8: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOP2 antigen affinity purified polyclonal antibody (Catalog # A04830-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOP2 at approximately 120 kDa. The expected band size for NOP2 is at 120 kDa.
![a04830 1 nop2 primary antibodies if testing 2 a04830 1 nop2 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a04830-1-nop2-primary-antibodies-if-testing-2.jpg)
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Figure 5. IF analysis of NOP2 using anti-NOP2 antibody (A04830-1) and anti-Beta Tubulin antibody (M01857-3).
NOP2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NOP2 Antibody (A04830-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a04830 1 nop2 primary antibodies fcm testing 3 a04830 1 nop2 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a04830-1-nop2-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of RT4 cells using anti-NOP2 antibody (A04830-1).
Overlay histogram showing RT4 cells stained with A04830-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOP2 Antibody (A04830-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NOP2 (Source: Uniprot.org, NCBI)
Gene Name
NOP2
Full Name
Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase
Weight
89.302kDa
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
NACHT, LRR and PYD domains-containing protein 2; Nucleotide-binding site protein 1; PYRIN domain and NACHT domain-containing protein 1; PYRIN-containing APAF1-like protein 2; NLRP2; NALP2; NBS1; PAN1; PYPAF2 NOP2 NOL1, NOP120, NSUN1, p120 NOP2 nucleolar protein probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase|NOL1/NOP2/Sun domain family, member 1|NOP2 nucleolar protein homolog|nucleolar protein 1, 120kDa|nucleolar protein 2 homolog|proliferating-cell nucleolar antigen p120|proliferation-associated nucleolar protein p120|putative ribosomal RNA methyltransferase NOP2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOP2, check out the NOP2 Infographic
![NOP2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOP2 Antibody Picoband® (A04830-1)
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