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Product Info Summary
| SKU: | M01607 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Neutrophil Elastase ELANE Rabbit Monoclonal Antibody
View all Neutrophil Elastase/ELA2 Antibodies
SKU/Catalog Number
M01607
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Neutrophil Elastase ELANE Rabbit Monoclonal Antibody catalog # M01607. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neutrophil Elastase ELANE Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01607)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOCI-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Neutrophil Elastase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01607 is reactive to ELANE in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
29 kDa
Calculated molecular weight
28518 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01607 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
FC 1:30
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Neutrophil Elastase using anti-Neutrophil Elastase antibody (M01607).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neutrophil Elastase antigen affinity purified monoclonal antibody (Catalog # M01607) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neutrophil Elastase at approximately 29 kDa. The expected band size for Neutrophil Elastase is at 29 kDa.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For ELANE (Source: Uniprot.org, NCBI)
Gene Name
ELANE
Full Name
Neutrophil elastase
Weight
28518 MW
Superfamily
peptidase S1 family
Alternative Names
Neutrophil elastase;3.4.21.37;Bone marrow serine protease;Elastase-2;Human leukocyte elastase;HLE;Medullasin;PMN elastase;ELANE;ELA2; ELANE ELA2, GE, HLE, HNE, NE, PMN-E, SCN1 elastase, neutrophil expressed neutrophil elastase|PMN elastase|bone marrow serine protease|elastase 2, neutrophil|elastase-2|granulocyte-derived elastase|leukocyte elastase|medullasin|polymorphonuclear elastase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ELANE, check out the ELANE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ELANE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neutrophil Elastase ELANE Rabbit Monoclonal Antibody (M01607)
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