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Product Info Summary
| SKU: | PB9744 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Neuroserpin/SERPINI1 Antibody Picoband®
View all Serpin I1/Neuroserpin Antibodies
SKU/Catalog Number
PB9744
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Neuroserpin/SERPINI1 Antibody Picoband® catalog # PB9744. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neuroserpin/SERPINI1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9744)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Neuroserpin, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9744 is reactive to SERPINI1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
46 kDa
Calculated molecular weight
46427 MW
Background of Serpin I1/Neuroserpin
Neuroserpin is a protein that in humans is encoded by the SERPINI1 gene. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9744 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, PANC Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Neuroserpin using anti-Neuroserpin antibody (PB9744).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug,
Lane 3: PANC Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neuroserpin antigen affinity purified polyclonal antibody (Catalog # PB9744) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neuroserpin at approximately 46 kDa. The expected band size for Neuroserpin is at 46 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SERPINI1 (Source: Uniprot.org, NCBI)
Gene Name
SERPINI1
Full Name
Neuroserpin
Weight
46427 MW
Superfamily
serpin family
Alternative Names
Neuroserpin;Peptidase inhibitor 12;PI-12;Serpin I1;SERPINI1;PI12; SERPINI1 PI12, neuroserpin serpin family I member 1 neuroserpin|PI-12|peptidase inhibitor 12|serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1|serpin I1|serpin peptidase inhibitor clade I member 1|serpin peptidase inhibitor, clade I (neuroserpin), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINI1, check out the SERPINI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neuroserpin/SERPINI1 Antibody Picoband® (PB9744)
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1 Customer Q&As for Anti-Neuroserpin/SERPINI1 Antibody Picoband®
Question
We are currently using anti-Neuroserpin/SERPINI1 antibody PB9744 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Z. Baker
Verified customer
Asked: 2015-07-03
Answer
The anti-Neuroserpin/SERPINI1 antibody (PB9744) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-07-03
