Product Info Summary
SKU: | A12035 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-NEURL4 Antibody Picoband®
SKU/Catalog Number
A12035
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NEURL4 Antibody Picoband® catalog # A12035. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NEURL4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12035)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NEURL4 recombinant protein (Position: E519-E1562).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12035 is reactive to NEURL4 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
185 kDa
Calculated molecular weight
166.907kDa
Background of NEURL4
The protein encoded by this gene is predicted and it includes two isoforms resulting from two alternatively spliced transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12035 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human 293T whole cell
IHC: human rectum adenocarcinoma tissue
FCM: U251 cell
Validation Images & Assay Conditions
![a12035 neurl4 primary antibodies wb testing 1 a12035 neurl4 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a12035-neurl4-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of NEURL4 using anti-NEURL4 antibody (A12035).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEURL4 antigen affinity purified polyclonal antibody (Catalog # A12035) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEURL4 at approximately 185 kDa. The expected band size for NEURL4 is at 167 kDa.
![a12035 neurl4 primary antibodies ihc testing 2 a12035 neurl4 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a12035-neurl4-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of NEURL4 using anti-NEURL4 antibody (A12035).
NEURL4 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEURL4 Antibody (A12035) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a12035 neurl4 primary antibodies fcm testing 3 a12035 neurl4 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a12035-neurl4-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of U251 cells using anti-NEURL4 antibody (A12035).
Overlay histogram showing U251 cells stained with A12035 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEURL4 Antibody (A12035, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For NEURL4 (Source: Uniprot.org, NCBI)
Gene Name
NEURL4
Full Name
Neuralized-like protein 4
Weight
166.907kDa
Alternative Names
Negative elongation factor B; NELF-B; Cofactor of BRCA1; NELFB; COBRA1; KIAA1182 NEURL4 neuralized E3 ubiquitin protein ligase 4 neuralized-like protein 4|neuralized homolog 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEURL4, check out the NEURL4 Infographic
![NEURL4 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEURL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEURL4 Antibody Picoband® (A12035)
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