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Product Info Summary
| SKU: | A10396-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NDUFA2 Antibody Picoband®
SKU/Catalog Number
A10396-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NDUFA2 Antibody Picoband® catalog # A10396-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NDUFA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10396-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NDUFA2 recombinant protein (Position: S7-A99).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10396-1 is reactive to NDUFA2 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
14 kDa
Calculated molecular weight
41409 MW
Background of NDUFA2
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 is a protein that in humans is encoded by the NDUFA2 gene. The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10396-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse heart tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NDUFA2 using anti-NDUFA2 antibody (A10396-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA2 antigen affinity purified polyclonal antibody (Catalog # A10396-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA2 at approximately 14 kDa. The expected band size for NDUFA2 is at 11 kDa.
Protein Target Info & Infographic
Gene/Protein Information For NDUFA2 (Source: Uniprot.org, NCBI)
Gene Name
NDUFA2
Full Name
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
Weight
41409 MW
Superfamily
complex I NDUFA2 subunit family
Alternative Names
Pre T-cell antigen receptor alpha; pT-alpha; pTa; pT-alpha-TCR; PTCRA NDUFA2 B8, CD14, CIB8, MC1DN13 NADH:ubiquinone oxidoreductase subunit A2 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2|NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa|NADH-ubiquinone oxidoreductase subunit CI-B8|complex I B8 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDUFA2, check out the NDUFA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDUFA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NDUFA2 Antibody Picoband® (A10396-1)
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