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Product Info Summary
| SKU: | A14016 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-N4BP2L2/Pfaap5 Antibody
SKU/Catalog Number
A14016
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-N4BP2L2/Pfaap5 Antibody (Catalog # A14016). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-N4BP2L2/Pfaap5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14016)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human N4BP2L2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14016 is reactive to N4BP2L2 in Human, Mouse
Reconstitution
Calculated molecular weight
67459 MW
Background of N4BP2L2
3 isoforms of the human protein are produced by alternative splicing.
Couch F.J., Genomics 36:86-99(1996).
Liu Y., Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14016 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from LOVO cells, using N4BP2L2 antibody A14016.
Protein Target Info & Infographic
Gene/Protein Information For N4BP2L2 (Source: Uniprot.org, NCBI)
Gene Name
N4BP2L2
Full Name
NEDD4-binding protein 2-like 2
Weight
67459 MW
Alternative Names
NEDD4-binding protein 2-like 2;Phosphonoformate immuno-associated protein 5;N4BP2L2;CG005, PFAAP5; N4BP2L2 92M18.3, CG005, CG016, PFAAP5 NEDD4 binding protein 2 like 2 NEDD4-binding protein 2-like 2|phosphonoformate immuno-associated protein 5|protein from BRCA2 region
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on N4BP2L2, check out the N4BP2L2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for N4BP2L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-N4BP2L2/Pfaap5 Antibody (A14016)
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No publications found for A14016
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-N4BP2L2/Pfaap5 Antibody
Question
I have a question about product A14016, anti-N4BP2L2/Pfaap5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14016 anti-N4BP2L2/Pfaap5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-11
Question
We are currently using anti-N4BP2L2/Pfaap5 antibody A14016 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
The anti-N4BP2L2/Pfaap5 antibody (A14016) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-01
Question
Would A14016 anti-N4BP2L2/Pfaap5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
As indicated on the product datasheet, A14016 anti-N4BP2L2/Pfaap5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-10
Question
Is this A14016 anti-N4BP2L2/Pfaap5 antibody reactive to the isotypes of N4BP2L2?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
The immunogen of A14016 anti-N4BP2L2/Pfaap5 antibody is Synthesized peptide derived from internal of human N4BP2L2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-23
Question
I have attached the WB image, lot number and protocol we used for corpus callosum using anti-N4BP2L2/Pfaap5 antibody A14016. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-05
