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Product Info Summary
| SKU: | PA1651 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MCM3 Antibody Picoband®
SKU/Catalog Number
PA1651
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MCM3 Antibody catalog # PA1651. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MCM3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1651)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MCM3, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1651 is reactive to MCM3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
91 kDa
Calculated molecular weight
90981 MW
Background of MCM3
MCM3 (MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 3), also called RLFB or P1 PROTEIN, is a protein that in humans is encoded by the MCM3 gene. MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The MCM3 gene is mapped to 6p12.2. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1651 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: NIH3T3 Whole Cell, HEPA Whole Cell
IHC: Human Lung Cancer Tissue, Rat Intestine Tissue, Rat Intestine Tissue, Mouse Intestine Tissue
ICC/IF: U20S cell
ICC: HELA Cell
FCM: SiHa cell
Validation Images & Assay Conditions
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Anti-MCM3 antibody, PA1651, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-MCM3 antibody, PA1651, IHC(P)
IHC(P): Rat Intestine Tissue
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Anti-MCM3 antibody, PA1651, ICC
ICC: HELA Cell
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Anti-MCM3 antibody, PA1651, Western blotting
All lanes: Anti MCM3 (PA1651) at 0.5ug/ml
Lane 1: NIH3T3 Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
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Anti-MCM3 antibody, PA1651, IHC(P)
IHC(P): Rat Intestine Tissue
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Anti-MCM3 antibody, PA1651, IHC(P)
IHC(P): Mouse Intestine Tissue
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Figure 7. IF analysis of MCM3 using anti-MCM3 antibody (PA1651).
MCM3 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCM3 Antibody (PA1651) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 8. Flow Cytometry analysis of SiHa cells using anti-MCM3 antibody (PA1651).
Overlay histogram showing SiHa cells stained with PA1651 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM3 Antibody (PA1651,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MCM3 (Source: Uniprot.org, NCBI)
Gene Name
MCM3
Full Name
DNA replication licensing factor MCM3
Weight
90981 MW
Superfamily
MCM family
Alternative Names
DNA replication licensing factor MCM3;3.6.4.12;DNA polymerase alpha holoenzyme-associated protein P1;P1-MCM3;RLF subunit beta;p102;MCM3; MCM3 HCC5, P1-MCM3, P1.h, RLFB minichromosome maintenance complex component 3 DNA replication licensing factor MCM3|DNA polymerase alpha holoenzyme-associated protein P1|DNA replication factor MCM3|MCM3 minichromosome maintenance deficient 3|RLF subunit beta|cervical cancer proto-oncogene 5|hRlf beta subunit|minichromosome maintenance deficient 3|p102|replication licensing factor, beta subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MCM3, check out the MCM3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MCM3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MCM3 Antibody Picoband® (PA1651)
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Customer Q&As
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5 Customer Q&As for Anti-MCM3 Antibody Picoband®
Question
Will anti-MCM3 antibody PA1651 work on bovine WB with testis?
R. Li
Verified customer
Asked: 2020-04-15
Answer
Our lab technicians have not validated anti-MCM3 antibody PA1651 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-MCM3 antibody PA1651 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-15
Question
Does PA1651 anti-MCM3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
You can see on the product datasheet, PA1651 anti-MCM3 antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-17
Question
We are currently using anti-MCM3 antibody PA1651 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The anti-MCM3 antibody (PA1651) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-18
Question
Is a blocking peptide available for product anti-MCM3 antibody (PA1651)?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
We do provide the blocking peptide for product anti-MCM3 antibody (PA1651). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-19
Question
My question regards to test anti-MCM3 antibody PA1651 on rat cervix carcinoma for research purposes, then I may be interested in using anti-MCM3 antibody PA1651 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Gonzalez
Verified customer
Asked: 2014-04-18
Answer
The products we sell, including anti-MCM3 antibody PA1651, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-04-18
