Product Info Summary
SKU: | MA1056 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-MAP1 Antibody (Monoclonal, HM-1)
SKU/Catalog Number
MA1056
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MAP1 Antibody (Monoclonal, HM-1) catalog # MA1056. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MAP1 Antibody (Monoclonal, HM-1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1056)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
HM-1
Isotype
Mouse IgG1
Immunogen
Rat brain microtubule-associated proteins (MAPs).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1056 is reactive to Map1a in Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
299531 MW
Background of MAP1A
Microtubules are the ubiquitous cytoskeletal structural components that are involved in intracellular transport. They are composed of tubulin and microtubule-associated proteins (MAPs). MAP1 is one of the major neuronal MAPs as well as being the largest (350KD). MAPs include MAP1A, MAP1B, and MAP2. MAP1a is a single-copy gene spanning 10.5 kb. MAP1a coding sequence is contained in five exons. MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1056 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Mouse, rat, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Mouse, rat, -
Western blot, 0.5-2μg/ml, Mouse, rat
Positive Control
IHC: Rat Brain Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-MAP1 antibody (monoclonal), MA1056, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info & Infographic
Gene/Protein Information For Map1a (Source: Uniprot.org, NCBI)
Gene Name
Map1a
Full Name
Microtubule-associated protein 1A
Weight
299531 MW
Superfamily
MAP1 family
Alternative Names
Microtubule-associated protein 1A;MAP-1A;MAP1A heavy chain;MAP1 light chain LC2;Map1a;Mtap1a; MAP1A MAP1L, MTAP1A microtubule associated protein 1A microtubule-associated protein 1A|MAP-1A|proliferation-related protein p80
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Map1a, check out the Map1a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Map1a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAP1 Antibody (Monoclonal, HM-1) (MA1056)
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Customer Q&As
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5 Customer Q&As for Anti-MAP1 Antibody (Monoclonal, HM-1)
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-MAP1 antibody (Monoclonal, HM-1) MA1056. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-10
Question
My question regarding product MA1056, anti-MAP1 antibody (Monoclonal, HM-1). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Brown
Verified customer
Asked: 2019-11-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1056 anti-MAP1 antibody (Monoclonal, HM-1), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-05
Question
We are currently using anti-MAP1 antibody (Monoclonal, HM-1) MA1056 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on primate tissues as well?
K. Brown
Verified customer
Asked: 2019-07-30
Answer
The anti-MAP1 antibody (Monoclonal, HM-1) (MA1056) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-30
Question
Will MA1056 anti-MAP1 antibody (Monoclonal, HM-1) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Miller
Verified customer
Asked: 2019-03-19
Answer
As indicated on the product datasheet, MA1056 anti-MAP1 antibody (Monoclonal, HM-1) as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-19
Question
Is this MA1056 anti-MAP1 antibody (Monoclonal, HM-1) reactive to the isotypes of MAP1A?
Verified Customer
Verified customer
Asked: 2018-05-14
Answer
The immunogen of MA1056 anti-MAP1 antibody (Monoclonal, HM-1) is Rat brain microtubule-associated proteins(MAPs). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-14