Product Info Summary
SKU: | A33313 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-LY6C Antibody Picoband®
SKU/Catalog Number
A33313
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LY6C Antibody Picoband® catalog # A33313. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LY6C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A33313)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse LY6C recombinant protein (Position: L27-G109).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A33313 is reactive to Ly6c1/2 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
14 kDa
Calculated molecular weight
14377 MW
Background of Ly-6C
Ly6C is a murine cell-surface antigen expressed by plasma cells, subsets of myeloid cells and many T cells, including memory T cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A33313 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
ELISA, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mouse RAW2647 whole cell, mouse ANA-1 whole cell
IHC: rat spleen tissue, mouse spleen tissue
FCM: mouse spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of LY6C using anti-LY6C antibody (A33313).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates,
Lane 2: mouse ANA-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LY6C antigen affinity purified polyclonal antibody (Catalog # A33313) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LY6C at approximately 14KD. The expected band size for LY6C is at 14KD.
Click image to see more details
Figure 2. IHC analysis of LY6C using anti-LY6C antibody (A33313).
LY6C was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LY6C Antibody (A33313) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. Flow Cytometry analysis of mouse spleen tissue using anti-LY6C antibody (A33313).
Overlay histogram showing mouse spleen tissue stained with A33313 (Blue line).The tissue was fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LY6C Antibody (A33313, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 4. IHC analysis of LY6C using anti-LY6C antibody (A33313).
LY6C was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LY6C Antibody (A33313) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ly6c1/2 (Source: Uniprot.org, NCBI)
Gene Name
Ly6c1/2
Full Name
Lymphocyte antigen 6C1
Weight
14377 MW
Alternative Names
NADH-ubiquinone oxidoreductase chain 2; NADH dehydrogenase subunit 2; Mtnd2; mt-Nd2; Nd2 Ly6c1|AA682074, AA959465, Ly-6, Ly-6C, Ly-6C1, Ly6, Ly6c|lymphocyte 6 complex, locus C1|lymphocyte 6C1|Lymphocyte Ly-6C|lymphocyte 6 complex, locus C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ly6c1/2, check out the Ly6c1/2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ly6c1/2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LY6C Antibody Picoband® (A33313)
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