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Product Info Summary
| SKU: | A17389-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-IRGQ Antibody Picoband®
SKU/Catalog Number
A17389-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IRGQ Antibody Picoband® catalog # A17389-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IRGQ Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17389-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human IRGQ recombinant protein (Position: D7-Q623). Human IRGQ shares 78.2% amino acid (aa) sequence identity with mouse IRGQ.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A17389-1 is reactive to IRGQ in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
62.717kDa
Background of IRGQ
IRGQ (Immunity Related GTPase Q) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include GTP binding.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17389-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A2780 whole cell, human 293T whole cell, human MCF-7 whole cell, human RT4 whole cell
FCM: HEL cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IRGQ using anti-IRGQ antibody (A17389-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A2780 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRGQ antigen affinity purified polyclonal antibody (Catalog # A17389-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRGQ at approximately 68 kDa. The expected band size for IRGQ is at 63 kDa.
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Figure 2. Flow Cytometry analysis of HEL cells using anti-IRGQ antibody (A17389-1).
Overlay histogram showing HEL cells stained with A17389-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRGQ Antibody (A17389-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For IRGQ (Source: Uniprot.org, NCBI)
Gene Name
IRGQ
Full Name
Immunity-related GTPase family Q protein
Weight
62.717kDa
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
Immunity-related GTPase family Q protein IRGQ FKSG271, IRGQ immunity related GTPase Q immunity-related GTPase family Q protein|immunity-related GTPase family, Q|immunity-related GTPase family, Q1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRGQ, check out the IRGQ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRGQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRGQ Antibody Picoband® (A17389-1)
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