Product Info Summary
SKU: | A33515 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-IRGM/Irgm1 Antibody Picoband®
SKU/Catalog Number
A33515
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IRGM/Irgm1 Antibody Picoband® catalog # A33515. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-IRGM/Irgm1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A33515)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse IRGM/Irgm1 recombinant protein (Position: E50-K333).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A33515 is reactive to Irgm1 in Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
14377 MW
Background of Irgm1
Immunity-related GTPase family M protein (IRGM), also known as interferon-inducible protein 1 (IFI1), is an enzyme that in humans is IRGM gene. This gene encodes a member of the p47 immunity-related GTPase family. The encoded protein may play a role in the innate immune response by regulating autophagy formation in response to intracellular pathogens. Polymorphisms that affect the normal expression of this gene are associated with a susceptibility to Crohn's disease and tuberculosis. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A33515 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
ELISA, 0.1-0.5 μg/ml, Mouse
Positive Control
WB: mouse RAW2647 whole cellmouse Ana-1 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of IRGM/Irgm1 using anti-IRGM/Irgm1 antibody (A33515).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse RAW264.7 whole cell lysates.
Lane 2: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRGM/Irgm1 antigen affinity purified polyclonal antibody (Catalog # A33515) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRGM/Irgm1 at approximately 45 kDa. The expected band size for IRGM/Irgm1 is at 45 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Irgm1 (Source: Uniprot.org, NCBI)
Gene Name
Irgm1
Full Name
Immunity-related GTPase family M protein 1
Weight
14377 MW
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
NADH-ubiquinone oxidoreductase chain 2; NADH dehydrogenase subunit 2; Mtnd2; mt-Nd2; Nd2 Irgm1|If, Ifggd3, Ifi1, Ii, Iigp3, Iipg3, Ir, Irgm, LRG-|immunity-related GTPase family M member 1|immunity-related GTPase family M protein 1|LPS-stimulated RAW 264.7 macrophage protein 47|immunity related GTPase m1|interferon-gamma-inducible GTPase Ifggd3 protein|interferon-inducible protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Irgm1, check out the Irgm1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Irgm1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRGM/Irgm1 Antibody Picoband® (A33515)
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