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Product Info Summary
| SKU: | PB9245 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IL18 Antibody Picoband®
View all IL-18/IL-1F4 Antibodies
SKU/Catalog Number
PB9245
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL18 Antibody Picoband® catalog # PB9245. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9245)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse IL-18 recombinant protein (Position: N36-S192). Mouse IL-18 shares 66% and 91% amino acid (aa) sequence identity with human and rat IL-18, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9245 is reactive to Il18 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
22135 MW
Background of IL-18/IL-1F4
Interleukin-18, also known as IL18, is a protein which in humans is encoded by the IL18 gene. IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. It is mapped to 11q22.2-q22.3. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12, it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-γ (IFN-γ) or type II interferon that plays an important role in activating the macrophages or other cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9245 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: NIH3T3 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-IL-18 Picoband antibody, PB9245, Western blotting
All lanes: Anti IL-18 (PB9245) at 0.5ug/ml
WB: Recombinant Mouse IL-18 Protein 0.5ng
Predicted bind size: 22KD
Observed bind size: 22KD
Click image to see more details
Anti-IL-18 Picoband antibody, PB9245, Western blotting
All lanes: Anti IL-18 (PB9245) at 0.5ug/ml
WB: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 50KD
Protein Target Info & Infographic
Gene/Protein Information For Il18 (Source: Uniprot.org, NCBI)
Gene Name
Il18
Full Name
Interleukin-18
Weight
22135 MW
Superfamily
IL-1 family
Alternative Names
Interleukin-18;IL-18;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;Il18;Igif; IL18 IGIF, IL-18, IL-1g, IL1F4 interleukin 18 interleukin-18|IFN-gamma-inducing factor|IL-1 gamma|iboctadekin|interleukin 18 (interferon-gamma-inducing factor)|interleukin-1 gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il18, check out the Il18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL18 Antibody Picoband® (PB9245)
Hello CJ!
PB9245 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang Yj, Shen Y, Chen Sh, Ge Xr. World J Gastroenterol. 2005 Aug 7;11(29):4524-9. Role Of Interleukin 18 In Acute Lung Inflammation Induced By Gut Ischemia Reperfusion.
Wang Y, Zhang X, Zhang Y, Xu H, Fang G. J Reprod Immunol. 2011 Dec;92(1-2):45-53. Doi: 10.1016/J.Jri.2011.07.002. Epub 2011 Sep 13. Expression And Localization Of Il-18 In The Hypothalamic-Pituitary-Ovarian Axis Of Non-Pregnant, Pregnant, And Abor...
Si Lf, Zhang Sy, Gao Cs, Chen Sl, Zhao J, Cheng Xc. Asian-Australas J Anim Sci. 2013 Oct;26(10):1399-405. Doi: 10.5713/Ajas.2013.13101. Effects Of Ifn-?? On Il-18 Expression In Pregnant Rats And Pregnancy Outcomes.
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-IL18 Antibody Picoband®
Question
Our lab want to know about to test anti-IL18 antibody PB9245 on mouse peripheral blood for research purposes, then I may be interested in using anti-IL18 antibody PB9245 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
The products we sell, including anti-IL18 antibody PB9245, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-13
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-IL18 antibody PB9245. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-12
Question
We are currently using anti-IL18 antibody PB9245 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
The anti-IL18 antibody (PB9245) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-07
Question
I see that the anti-IL18 antibody PB9245 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-19
Question
Will anti-IL18 antibody PB9245 work for WB with peripheral blood?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
According to the expression profile of peripheral blood, IL18 is highly expressed in peripheral blood. So, it is likely that anti-IL18 antibody PB9245 will work for WB with peripheral blood.
Boster Scientific Support
Answered: 2019-06-27
Question
I was wanting to use your anti-IL18 antibody for WB for mouse peripheral blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse peripheral blood identification?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
You can see on the product datasheet, PB9245 anti-IL18 antibody has been validated for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse peripheral blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-12
Question
We have been able to see staining in mouse liver. Do you have any suggestions? Is anti-IL18 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
From literature liver does express IL18. From Uniprot.org, IL18 is expressed in lower esophagus mucosa, liver, urinary bladder, peripheral blood, among other tissues. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-30
Question
I have a question about product PB9245, anti-IL18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9245 anti-IL18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-14
Question
See below the WB image, lot number and protocol we used for peripheral blood using anti-IL18 antibody PB9245. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-07
Question
Our lab want to know about using your anti-IL18 antibody for positive regulation of nf-kappab transcription factor activity studies. Has this antibody been tested with western blotting on nih3t3 whole cell lysate? We would like to see some validation images before ordering.
A. Krishna
Verified customer
Asked: 2018-04-11
Answer
We appreciate your inquiry. This PB9245 anti-IL18 antibody is validated on nih3t3 whole cell lysate. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-04-11
Question
Do you have a BSA free version of anti-IL18 antibody PB9245 available?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL18 antibody PB9245 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-20
Question
Our team were satisfied with the WB result of your anti-IL18 antibody. However we have been able to see positive staining in lower esophagus mucosa secreted. using this antibody. Is that expected? Could you tell me where is IL18 supposed to be expressed?
K. Baker
Verified customer
Asked: 2015-12-01
Answer
According to literature, lower esophagus mucosa does express IL18. Generally IL18 expresses in secreted. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-12-01
Question
Is a blocking peptide available for product anti-IL18 antibody (PB9245)?
S. Singh
Verified customer
Asked: 2015-03-04
Answer
We do provide the blocking peptide for product anti-IL18 antibody (PB9245). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-03-04
Question
Will PB9245 anti-IL18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Patel
Verified customer
Asked: 2014-08-04
Answer
You can see on the product datasheet, PB9245 anti-IL18 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-08-04
Question
Is this PB9245 anti-IL18 antibody reactive to the isotypes of IL18?
S. Roberts
Verified customer
Asked: 2013-10-22
Answer
The immunogen of PB9245 anti-IL18 antibody is E.coli-derived mouse IL-18 recombinant protein (Position: N36-S192). Mouse IL-18 shares 66% and 91% amino acid (aa) sequence identity with human and rat IL-18, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-10-22
