Anti-Human CYP1A1 DyLight® 488 conjugated Antibody

SKU A00471-Dyl488
Size 100ug/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Applications Flow Cytometry

Overview

Product Name Anti-Human CYP1A1 DyLight® 488 conjugated Antibody
SKU/Catalog Number A00471-Dyl488
Storage & Handling At 2-8°C for one year. Protect from light. Do not freeze.
Size 100ug/vial
Description Rabbit IgG Polyclonal Anti-Human CYP1A1 Antibody DyLight® 488 Conjugated, Flow Validated.
Cite This Product Anti-Human CYP1A1 DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00471-Dyl488)
Host Rabbit
Contents/Buffer Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Form Liquid
Immunogen E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Flow Cytometry, 1-3μg/1x106 cells

Images And Assay Conditions

Figure 1. Flow Cytometry analysis of K562 cells using anti-Human CYP1A1 antibody (A00471-Dyl488).
Overlay histogram showing K562 cells stained with A00471-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with r

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P04798
Gene Name CYP1A1
Protein Name cytochrome P450, family 1, subfamily A, polypeptide 1
Tissue Specificity Lung, lymphocytes and placenta.
Alternative Names Cytochrome P450 1A1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; CYP1A1
Subcellular Localization Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Research Areas Human

*You can search these to find other products in these research areas.
Background CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline). It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo(a)pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7,8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7,8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7,8-dihydrodiol-9,10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo(a)pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo(a)pyrene carcinogen into the systemic circulation.

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In stock
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A00471-Dyl488
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$290.00

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Publications

Glutathione S-Transferases and Cytochrome P450 enzyme expression in patients with Intracranial Tumors: Preliminary Report of 55 Patients
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