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Product Info Summary
| SKU: | FC00344 |
|---|---|
| Size: | 100ug, 500ug |
| Reactive Species: | Cynomolgus monkey, Human, Rhesus monkey |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated
SKU/Catalog Number
FC00344
Size
100ug, 500ug
Form
Liquid
Description
Boster Bio Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated (Catalog# FC00344). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00344)
Host
Mouse
Contents
PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: OKT4
Isotype
IgG2b,k
Immunogen
Human peripheral blood T lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00344 is reactive to CD4 in Cynomolgus monkey, Human, Rhesus monkey
Reconstitution
Calculated molecular weight
51111 MW
Background of CD4
CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00344 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocyte gated PBMCs stained with purified anti-human CD4 (clone OKT-4) followed by Goat anti-mouse IgG (H+L) Alexa Fluor 488 (red histogram). Lymphocyte gated PBMCs stained with only Goat anti-mouse IgG (H+L) Alexa Fluor 488 (Blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For CD4 (Source: Uniprot.org, NCBI)
Gene Name
CD4
Full Name
T-cell surface glycoprotein CD4
Weight
51111 MW
Alternative Names
T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;CD4;CD4; CD4 CD4mut CD4 molecule T-cell surface glycoprotein CD4|CD4 (p55)|CD4 receptor|T-cell surface T4/Leu-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD4, check out the CD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated (FC00344)
Hello CJ!
FC00344 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
Ouyang W, An T, Guo D, Wu S, Tien P. Aids Res Hum Retroviruses. 2013 Mar;29(3):613-20. Doi: 10.1089/Aid.2012.0059. Epub 2013 Jan 11. The Potent Human Immunodeficiency Virus Type 1 (Hiv-1) Entry Inhibitor Hr212 Blocks Formation Of The Envelope Glyc...
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated
Question
I have a question about product FC00344, anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Jones
Verified customer
Asked: 2020-03-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00344 anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-12
Question
Our lab want to know about to test anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated FC00344 on human pancreas for research purposes, then I may be interested in using anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated FC00344 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The products we sell, including anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated FC00344, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-05
Question
Does anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated FC00344 work for Flow Cytometry with pancreas?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
According to the expression profile of pancreas, CD4 is highly expressed in pancreas. So, it is likely that anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated FC00344 will work for Flow Cytometry with pancreas.
Boster Scientific Support
Answered: 2020-02-10
Question
See attached the WB image, lot number and protocol we used for pancreas using anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated FC00344. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-18
Question
Is there a BSA free version of anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated FC00344 available?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated FC00344 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-04
Question
Would FC00344 anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
It shows on the product datasheet, FC00344 anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-26
Question
Is this FC00344 anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated reactive to the isotypes of CD4?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
The immunogen of FC00344 anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated is Human peripheral blood T lymphocytes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-31
Question
I was wanting to use your anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated for Flow Cytometry for human pancreas on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreas identification?
Verified Customer
Verified customer
Asked: 2017-12-06
Answer
As indicated on the product datasheet, FC00344 anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated FC00344. Let me know if you need anything else.
G. Huang
Verified customer
Asked: 2017-07-04
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-04
Question
Is a blocking peptide available for product anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated (FC00344)?
P. Anderson
Verified customer
Asked: 2016-03-28
Answer
We do provide the blocking peptide for product anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated (FC00344). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-03-28
Question
I see that the anti-human CD4 Monoclonal antibody Unconjugated, Flow Validated FC00344 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
A. Parker
Verified customer
Asked: 2015-11-26
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-11-26
