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Product Info Summary
| SKU: | A13079-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-HTRA4 Antibody Picoband®
SKU/Catalog Number
A13079-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HTRA4 Antibody Picoband® catalog # A13079-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HTRA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13079-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human HTRA4 recombinant protein (Position: A31-D464). Human HTRA4 shares 68.7% and 68.9% amino acid (aa) sequence identity with mouse and rat HTRA4, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A13079-1 is reactive to HTRA4 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
51 kDa
Calculated molecular weight
50.979kDa
Background of HTRA4
This gene encodes a member of the HtrA family of proteases. The encoded protein contains a putative signal peptide, an insulin growth factor binding domain, a Kazal protease inhibitor domain, a conserved trypsin domain and a PDZ domain. Based on studies on other related family members, this enzyme may function as a secreted oligomeric chaperone protease to degrade misfolded secretory proteins. Other human HtrA proteins have been implicated in arthritis, tumor suppression, unfolded stress response, apoptosis, and aging.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13079-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human PC-3 whole cell, human THP-1 whole cell, human Hela whole cell
FCM: Daudi cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HTRA4 using anti-HTRA4 antibody (A13079-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTRA4 antigen affinity purified polyclonal antibody (Catalog # A13079-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTRA4 at approximately 51 kDa. The expected band size for HTRA4 is at 51 kDa.
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Figure 2. Flow Cytometry analysis of Daudi cells using anti-HTRA4 antibody (A13079-1).
Overlay histogram showing Daudi cells stained with A13079-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-HTRA4 Antibody (A13079-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For HTRA4 (Source: Uniprot.org, NCBI)
Gene Name
HTRA4
Full Name
Serine protease HTRA4
Weight
50.979kDa
Superfamily
peptidase S1C family
Alternative Names
EC 3.4.21; EC 3.4.21.-; EC 3.4.21.108; FLJ90724; HTRA 4; HtrA serine peptidase 4; HTRA4; probable serine protease HTRA4 HTRA4 HtrA serine peptidase 4 serine protease HTRA4|high-temperature requirement factor A4|probable serine protease HTRA4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HTRA4, check out the HTRA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HTRA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HTRA4 Antibody Picoband® (A13079-1)
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